"
August/6 August 2009
From 2009.igem.org
(Difference between revisions)
(→To do in lab) |
(→Design genetic circuits) |
||
| Line 29: | Line 29: | ||
We take notice of signal molecules. So I put them in order in the chart below.<br/> | We take notice of signal molecules. So I put them in order in the chart below.<br/> | ||
*Inducer ⇒（synthesize) signal moleculer ⇒（synthesize) Receiver | *Inducer ⇒（synthesize) signal moleculer ⇒（synthesize) Receiver | ||
| - | * LuxI ⇒ 3OC6HSL ⇒ LuxR | + | * LuxI ⇒ 3OC6HSL ⇒ LuxR → positive regulation |
| - | * LasI ⇒ AI-1(3OC12HSL) | + | * LasI ⇒ AI-1(3OC12HSL) ⇒ LasR → positive regulation |
| - | * CinI ⇒ 3OH,C14:1-HSl | + | * CinI ⇒ 3OH,C14:1-HSl ⇒ CinR →positive regulation |
| - | * RhlI ⇒ AI-1(C4HSL) ⇒ RhlR | + | * RhlI ⇒ AI-1(C4HSL) ⇒ RhlR → positive regulation |
* （exception）agrD synthesize AIP with agrB.AIp synthesize agrC . AgrA which is synthesized by agrC activate promoter. | * （exception）agrD synthesize AIP with agrB.AIp synthesize agrC . AgrA which is synthesized by agrC activate promoter. | ||
Revision as of 23:58, 6 August 2009
Contents |
Morning Meeting
To do in lab
1. Transform & Selection
- Medium buildng
- LB
- LB Amp+
- LB Kan+
- Transformation
- About 16 kinds of parts
We transform these following 14 parts today.
B0015 Plate 1 23L S03878 Plate2 16M
C0179 Pate 2 8M C0070 Plate2 12H
F1610 Plate2 24G B0034 Plate1 2M
I0462 Plate1 8O C0078 Plate1 14D
C0077 Plate1 14A I1466 Plate1 23J
2. Breeding
3. Refinement
To bring
- Timer
- Pen
- Slipper
Design genetic circuits
We take notice of signal molecules. So I put them in order in the chart below.
*Inducer ⇒（synthesize) signal moleculer ⇒（synthesize) Receiver
* LuxI ⇒ 3OC6HSL ⇒ LuxR → positive regulation * LasI ⇒ AI-1(3OC12HSL) ⇒ LasR → positive regulation * CinI ⇒ 3OH,C14:1-HSl ⇒ CinR →positive regulation * RhlI ⇒ AI-1(C4HSL) ⇒ RhlR → positive regulation * （exception）agrD synthesize AIP with agrB.AIp synthesize agrC . AgrA which is synthesized by agrC activate promoter.
From now on,We have to do what I mention below
1.Search other cell-cell comunication system
2.Check systems component completely
3.Create new systems or reinforce cell funcution with additional ideas
4.Search the effect of cross talk
written by Tadasi Nakamura
