Team:KULeuven/10 August 2009

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Revision as of 08:23, 10 August 2009

Project progress

Progress of parts

[edit] Blue Light Receptor

PCR reaction with the new primers 2260 and 2261 to get the exact blue light promotor sequence. The reaction was succesful and with no byproducts, so a simple PCR clean up was executed.

  • concentration of clean pcr product.:
    • 102,6 ng/µl
    • 1,88

[edit] Vanillin Production

  • Ligated genes are electrophoresed.
  • Reorderd part (COMT + RBS) from the registry.
  • Also we realized the proteins need to be degraded fast for the control mechanism to work, so a ssRA degradation tag like ANDENYALVA or ANDENYALAA is needed.
    • However since some genes have just been ligated we will only tag sam5, COMT and the ech gene.

[edit] Vanillin Receptor

  • There was no result from our setup on Friday. We think there was an error with the protocol because we didn't shake before incubation. So we did a new transformation of every gene.

[edit] Key/Lock/Anti-Key

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