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Uppsala-Sweden/8 August 2009
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(New page: ==Observation Transformation== The transformation were observerd on 2pm. Colonies were found for adh2(Z), ADH2(Y) and pirB on the concentrated plates (1300µl spin down discard 900µl, re...)
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(New page: ==Observation Transformation== The transformation were observerd on 2pm. Colonies were found for adh2(Z), ADH2(Y) and pirB on the concentrated plates (1300µl spin down discard 900µl, re...)
Newer edit →
Revision as of 09:38, 10 August 2009
Observation Transformation
The transformation were observerd on 2pm. Colonies were found for adh2(Z), ADH2(Y) and pirB on the concentrated plates (1300µl spin down discard 900µl, resuspend (200µl) and plated)
Plates without colonies were reincubated at 37°C to check them the next day for potential colonies.
Transfer to LB
Single colonies were picked and inoculated in 5ml LB at 37°C at 250 RPM.
