Revision as of 14:31, 10 August 2009

Used Parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=KULeuven Submitted Parts]

Blue light receptor

part	plasmid	description	status	μl left	concentration	date
MC4100	/	strain of E.coli				july 24
2171	/	forward primer for		90µl	20μM	july 24
2172	/	reverse primer for		90µl	20μM	july 24
2171	/	forward primer for			100μM	july 24
2172	/	reverse primer for			100μM	july 24
2260	/	forward primer for		45µl	20μM	aug 10
2261	/	reverse primer for		45µl	20μM	aug 10
2260	/	forward primer for			100μM	aug 10
2261	/	reverse primer for			100μM	aug 10
	pSB1A2	GFP + rbs + terminator	purified			july 29
	pSB1A2	GFP + rbs + terminator	restricted with EcoRI and XbaI			july 29
	/	BLR promoter region	restricted with EcoRI			july 29
	/	BLR promoter region	4 microcentrifuge tubes			july 30
	/	BLR promoter region	1 microcentrifuge tube	28μl	conc 102,6 ng/μl	aug 10

Key/antikey/lock

part	plasmid	description	status	μl left	concentration	date
	/	GFP with LVA tag	purified			july 28
	/	tetR promoter	purified			july 28

vanillin synthesis

plasmid	description	status	concentration	date
pSB1A2	ech + rbs	purified plasmid	108,8 ng/µl	August 4
pSB1A2	fcs + rbs	purified plasmid	113,9 ng/µl	August 4
pSB1A2	Sam5 + rbs	received from Edinburgh		August 3
pSB1A2	Sam5 + rbs, PstI site removed	purified plasmid	101,3 ng/µl	August 5
pSB1A2	Sam8 + rbs	purified plasmid	95,7 ng/µl	August 5
pSB1A2	Sam8	purified plasmid	81,5 ng/µl	August 5
pSB1A2	COMT	purified plasmid	122,9 ng/µl	August 5
pSB1A2	COMT + rbs	plasmid from registry		August 6
pSB1A2	ech + rbs	purified digest	16,9 ng/µl	August 6
	fcs + rbs	purified digest	16,6 ng/µl	August 6
pSB1A2	sam5 + rbs	purified digest	3,1 ng/µl	August 7
	sam8 + rbs	purified digest	3,4 ng/µl	August 7

vanillin receptor

part	plasmid	description	status	μl left	concentration	date

|- |||pSB1A2||ech + rbs||purified plasmid||||108,8 ng/µl||August 4 |- |||pSB1A2||fcs + rbs||purified plasmid||||113,9 ng/µl||August 4 |- |||pSB1A2||Sam5 + rbs||received from Edinburgh||||||August 3 |- |||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid||||101,3 ng/µl||August 5

miscellaneous

part	plasmid	description	μl left	concentration	date
dNTP mix		ATP, GTP, TTP, CTP	94	10μM	aug 10
	pSB1A2	medium constitutieve promoter			july 23
ampicilline

@@ Line 39: / Line 39: @@
 | {{kulpart|BBa_K238000}}||/||BLR promoter region||4 microcentrifuge tubes||||||july 30
 |-
-| {{kulpart|BBa_K238013}}||/||BLR promoter region||1 microcentrifuge tube|||28μl|| conc 102,6 ng/μl|||||aug 10
+| {{kulpart|BBa_K238013}}||/||BLR promoter region||1 microcentrifuge tube|||28μl|| conc 102,6 ng/μl|||aug 10
 |}

Team:KULeuven/Freezer

From 2009.igem.org