Team:Virginia/1 June 2009

From 2009.igem.org

(Difference between revisions)
(New page: Used the total genome extract from friday to do PCR run with ArsC primers. *used low concentration of MgCl .7 mM.)
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Used the total genome extract from friday to do PCR run with ArsC primers.
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==Overnight Results==
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*used low concentration of MgCl .7 mM.
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*All plates had colonies
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**Including negative control
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**BFP + B0032 assembly had few colonies
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==Today==
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*Ran PCR of E.coli total genomic isolate with primers for ArsC
 +
**Used the total genome extract from friday to do PCR run with ArsC primers.
 +
**used low concentration of MgCl .7 mM.

Revision as of 18:10, 1 June 2009

Overnight Results

  • All plates had colonies
    • Including negative control
    • BFP + B0032 assembly had few colonies

Today

  • Ran PCR of E.coli total genomic isolate with primers for ArsC
    • Used the total genome extract from friday to do PCR run with ArsC primers.
    • used low concentration of MgCl .7 mM.
Retrieved from "http://2009.igem.org/Team:Virginia/1_June_2009"