Team:Virginia/7 August 2009

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(Difference between revisions)
m (Today)
 
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*Growth curve at .1 mM
*Growth curve at .1 mM
*Transformed DB3.1 with pSB3K3
*Transformed DB3.1 with pSB3K3
-
*Gel of PCR with new GFP primers for 76 & 82 (lanes 2+3)
+
*Gel of PCR with new GFP primers for 76 & 82 (lanes 2+3), ABP+HA PCR (HA2) in lane 1
**[[Image:Vgem080709.jpg]]
**[[Image:Vgem080709.jpg]]
*Result of PCR to add HA tag to PCS:
*Result of PCR to add HA tag to PCS:
**[[Image:Vgem080709-PCS_HA2.jpg]]
**[[Image:Vgem080709-PCS_HA2.jpg]]
**Too many bands, smears, need to redo
**Too many bands, smears, need to redo

Latest revision as of 18:48, 12 August 2009

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Today

  • Growth curve at .1 mM
  • Transformed DB3.1 with pSB3K3
  • Gel of PCR with new GFP primers for 76 & 82 (lanes 2+3), ABP+HA PCR (HA2) in lane 1
    • Vgem080709.jpg
  • Result of PCR to add HA tag to PCS:
    • Vgem080709-PCS HA2.jpg
    • Too many bands, smears, need to redo