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Team:Virginia/7 August 2009
From 2009.igem.org
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*Growth curve at .1 mM | *Growth curve at .1 mM | ||
*Transformed DB3.1 with pSB3K3 | *Transformed DB3.1 with pSB3K3 | ||
| - | *Gel of PCR with new GFP primers for 76 & 82 (lanes 2+3) | + | *Gel of PCR with new GFP primers for 76 & 82 (lanes 2+3), ABP+HA PCR (HA2) in lane 1 |
**[[Image:Vgem080709.jpg]] | **[[Image:Vgem080709.jpg]] | ||
*Result of PCR to add HA tag to PCS: | *Result of PCR to add HA tag to PCS: | ||
**[[Image:Vgem080709-PCS_HA2.jpg]] | **[[Image:Vgem080709-PCS_HA2.jpg]] | ||
**Too many bands, smears, need to redo | **Too many bands, smears, need to redo | ||
Latest revision as of 18:48, 12 August 2009
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Today
- Growth curve at .1 mM
- Transformed DB3.1 with pSB3K3
- Gel of PCR with new GFP primers for 76 & 82 (lanes 2+3), ABP+HA PCR (HA2) in lane 1
- Result of PCR to add HA tag to PCS:
- Too many bands, smears, need to redo
