Template:Team:KULeuven/13 August 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
(New page: * Miniprep on Sam8 and Ech *)
Line 1: Line 1:
-
* Miniprep on Sam8 and Ech
+
# Plated ComT (+RBS) from the registry
-
*
+
#* Growing overnight in 37C
 +
# Plated Ech
 +
#* From newly made liquid culture
 +
# Miniprep of Ech and Sam8
 +
Nanodrop results:
 +
{| border ="1" align="center"
 +
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ
 +
|- align="center"
 +
| Ech ||72,7 || 1,94 ||
 +
|- align="center"
 +
| Sam8 ||66,2|| 2,00 || 
 +
|-
 +
|}
 +
# Restriction digest on Ech and Sam8
 +
# Gelelectrophorese on Ech and Sam8
 +
# New electrocompetent cells were used for the electroporation using the old ligations
 +
#* Lig A -> Sam5 + Sam8
 +
#* Lig C -> Ech + fcs
 +
#* Note: there was no spark
 +
# Plating of the electroporated cells
 +
#* 200 μl per plate twice
 +
#* 600 μl left of A and C

Revision as of 15:30, 13 August 2009

  1. Plated ComT (+RBS) from the registry
    • Growing overnight in 37C
  2. Plated Ech
    • From newly made liquid culture
  3. Miniprep of Ech and Sam8

Nanodrop results:

Part concentration (ng/μl) 260/280 λ 260/230 λ
Ech 72,7 1,94
Sam8 66,2 2,00
  1. Restriction digest on Ech and Sam8
  2. Gelelectrophorese on Ech and Sam8
  3. New electrocompetent cells were used for the electroporation using the old ligations
    • Lig A -> Sam5 + Sam8
    • Lig C -> Ech + fcs
    • Note: there was no spark
  4. Plating of the electroporated cells
    • 200 μl per plate twice
    • 600 μl left of A and C
Retrieved from "http://2009.igem.org/Template:Team:KULeuven/13_August_2009/VanillinProduction"