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Team:Kyoto/Calendar/24 July 2009
From 2009.igem.org
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====Title==== | ====Title==== | ||
{| class="notebook" | {| class="notebook" | ||
| - | |''' | + | |'''24 July: Miniprep Kit, Restriction Enzyme Digestion Electrophoresis''' |
|} | |} | ||
| Line 19: | Line 19: | ||
{| class="notebook" | {| class="notebook" | ||
| | | | ||
| - | #Miniprep Kit | + | #[[#Miniprep|Miniprep Kit]] |
#Restriction Enzyme Digestion | #Restriction Enzyme Digestion | ||
| - | #Electrophoresis | + | #[[#Electrophoresis|Electrophoresis]] |
|} | |} | ||
====Storage list==== | ====Storage list==== | ||
| Line 40: | Line 40: | ||
'''Protocol''' | '''Protocol''' | ||
*[3-O-2, 3-A-4] | *[3-O-2, 3-A-4] | ||
| - | #Cultured samples (090722 9:30 | + | #Cultured samples (090722 9:30 - 090723 16:50) |
| - | #Centrifuged | + | #Centrifuged 2 mins at 15000 G 4 °C. |
#Removed the top of layer. | #Removed the top of layer. | ||
| - | #Centrifuged | + | #Centrifuged 2 mins at 15000 G 4 °C. |
#Removed the top of layer. | #Removed the top of layer. | ||
| - | #Centrifuged | + | #Centrifuged 10 mins at 14000 G 4 °C. |
#Applied the top of layer to column. | #Applied the top of layer to column. | ||
| - | #Centrifuged RT, | + | #Centrifuged RT, 10 sec and remove through. |
| - | #Added Buffer PB | + | #Added Buffer PB 500 µl. |
| - | #Centrifuged RT, | + | #Centrifuged RT, 10 sec and remove through. |
| - | #Added Buffer PE | + | #Added Buffer PE 650 µl. |
| - | #Centrifuged RT, | + | #Centrifuged RT, 10 sec and remove through. |
| - | #Centrifuged 1min at | + | #Centrifuged 1min at 17900 G 4 °C. |
#Exchanged tube. (remove Ethanol) | #Exchanged tube. (remove Ethanol) | ||
| - | #Added Milli Q | + | #Added Milli Q 50 µl RT 1min. |
| - | #Centrifuged 1min at | + | #Centrifuged 1min at 17900 G 4°C. |
#Collected through and measured concentration. | #Collected through and measured concentration. | ||
---- | ---- | ||
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!12.7!!12.7!!12.9!!12.9 | !12.7!!12.7!!12.9!!12.9 | ||
|} | |} | ||
| - | Incubated at 37 | + | Incubated at 37 °C (090724 12:50 to 13:20 ) |
{| class="data" | {| class="data" | ||
! !!1!!2!!3!!4!!5 | ! !!1!!2!!3!!4!!5 | ||
Revision as of 05:05, 14 August 2009
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Lab-note on 24 July 2009
| Experimenter | Kashima, Takashima, Hibino, Miyazawa |
| Note-taker | Takashima |
Title
| 24 July: Miniprep Kit, Restriction Enzyme Digestion Electrophoresis |
Main Protocol
|
Storage list
| Sample Date | Sample Name | Enzyme | purification Kit | Product | Storage |
|---|---|---|---|---|---|
| iGEM DNA parts kit | 3-O-2 | Eco R1 | 6F freezer | ||
| iGEM DNA parts kit | 3-A-4 | Eco R1 | 6F freezer | ||
| iGEM DNA parts kit | 3-O-2 | Eco R1 PST1 | 6F freezer | ||
| iGEM DNA parts kit | 3-A-4 | Eco R1 PST1 | 6F freezer |
Miniprep
|
Protocol
Results
|
Electrophoresis
|
Protocol
Incubated at 37 °C (090724 12:50 to 13:20 )
Results 
Discussion
|
