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Team:Newcastle/Labwork/14 August 2009
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(New page: =Lab 14/08/09= ==Metal Sensor Team== ===Summary=== So far we have shown that our ''Bacillus subtilis 168'' cultures, once the ''GFP-rrnb'' DNA has been added, can survive on LB + Chloramp...)
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Revision as of 10:34, 14 August 2009
Lab 14/08/09
Metal Sensor Team
Summary
So far we have shown that our Bacillus subtilis 168 cultures, once the GFP-rrnb DNA has been added, can survive on LB + Chloramphenicol media. This suggests that we have successfully transformed our B. subtilis cells as the wild type B. subtilis cells do not have this resistance. However we need to prove that the resistance inherited by the Bacillus subtilis bacteria is due to the GFP-rrnb and not acquired by other means. Today, we intend to pour some LB + Chloramphenicol + starch plates and then plate our 'transformed' Bacillus cells onto these plates. The reason for adding starch to the plates: the GFP-rrnb plasmid removes the Bacillus subtilis bacteria's ability to break down starch using the amyE enzyme - starch plates will prove the bacteria have been transformed.
