Template:Team:KULeuven/13 August 2009/VanillinProduction
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#* From newly made liquid culture | #* From newly made liquid culture | ||
# Miniprep of Ech and Sam8 | # Miniprep of Ech and Sam8 | ||
- | #* Nanodrop results:{| border ="1" align="center" | + | #* Nanodrop results: |
+ | #: {| border ="1" align="center" | ||
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ | !| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ | ||
|- align="center" | |- align="center" |
Revision as of 12:38, 14 August 2009
- Plated ComT (+RBS) from the registry
- Growing overnight in 37C
- Plated Ech
- From newly made liquid culture
- Miniprep of Ech and Sam8
- Nanodrop results:
- {| border ="1" align="center"
!| Part || concentration (ng/μl) || 260/280 λ || 260/230 λ |- align="center" | Ech ||72,7 || 1,94 || |- align="center" | Sam8 ||66,2|| 2,00 || |- |}
- Restriction digest on Ech and Sam8
- Gelelectrophorese on Ech and Sam8
- New electrocompetent cells were used for the electroporation using the old ligations
- Lig A -> Sam5 + Sam8
- Lig C -> Ech + fcs
- Note: there was no spark
- Plating of the electroporated cells
- 200 μl per plate twice
- 600 μl left of A and C