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EPF-Lausanne/14 August 2009
From 2009.igem.org
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==Wet Lab== | ==Wet Lab== | ||
| + | Miniprep of the clones which contained our 2nd read-out system. | ||
| + | Synthesis of the Trp operon sequence with Klenow fragment extension, purification, digestion, purification again and ligation during 1 hour at RT. Then, transformation of DH5-alpha cells with the ligation products, plates incubated overnight at 37°C. | ||
| + | |||
| + | Ligation during 1 hour at RT with the backbones obtained by gel extraction yesterday evening. | ||
| + | |||
| + | Transformation of DH5-alpha cells with a whole bunch of ligation products: LovTap, LovTap-Term, LacI-RBS-LovTap-Term, LacLP-RBS-LovTap-Term, T7-RBS-LovTap-Term, read-out 2 (total of 28 plates!!!!). | ||
| + | |||
| + | Plates left to incubate overnight at 37°C. | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 13:13, 14 August 2009
Contents |
Wet Lab
Miniprep of the clones which contained our 2nd read-out system.
Synthesis of the Trp operon sequence with Klenow fragment extension, purification, digestion, purification again and ligation during 1 hour at RT. Then, transformation of DH5-alpha cells with the ligation products, plates incubated overnight at 37°C.
Ligation during 1 hour at RT with the backbones obtained by gel extraction yesterday evening.
Transformation of DH5-alpha cells with a whole bunch of ligation products: LovTap, LovTap-Term, LacI-RBS-LovTap-Term, LacLP-RBS-LovTap-Term, T7-RBS-LovTap-Term, read-out 2 (total of 28 plates!!!!).
Plates left to incubate overnight at 37°C.
People in the lab
Nath, Basile, Gab, Christian
