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Team:Todai-Tokyo/Notebook/bioclock
From 2009.igem.org
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2. Make a gene network that express oscillatory pattern, using these genes.<BR> | 2. Make a gene network that express oscillatory pattern, using these genes.<BR> | ||
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| + | =='''7/7'''== | ||
| + | Cloning the parts<Br> | ||
preculture of the Biobrick parts for Miniprep<BR> | preculture of the Biobrick parts for Miniprep<BR> | ||
| - | =='''7/8'''==<Br> | + | =='''7/8'''== |
| - | + | Cloning the parts<Br> | |
Miniprep of E.coli cells containing Biobrick parts with Promega, Wizard Plus SV Miniprep DNA Purification System<BR> | Miniprep of E.coli cells containing Biobrick parts with Promega, Wizard Plus SV Miniprep DNA Purification System<BR> | ||
failure | failure | ||
| - | =='''7/9'''==<Br> | + | =='''7/9'''== |
| - | + | Cloning the parts<Br> | |
Miniprep again of E.coli cells containing Biobrick parts with Promega, Wizard Plus SV Miniprep DNA Purification System<BR> | Miniprep again of E.coli cells containing Biobrick parts with Promega, Wizard Plus SV Miniprep DNA Purification System<BR> | ||
Revision as of 02:41, 16 August 2009
Contents |
Plan
Aim: Create E.coli that show oscillatory gene expression pattern
Methods:
1. Clone the following genes from Bacillus subtilis into a biobrick vector.
- plate1,13B
- plate1,13L
- plate2,1H
2. Make a gene network that express oscillatory pattern, using these genes.
7/7
Cloning the parts
preculture of the Biobrick parts for Miniprep
7/8
Cloning the parts
Miniprep of E.coli cells containing Biobrick parts with Promega, Wizard Plus SV Miniprep DNA Purification System
failure
7/9
Cloning the parts
Miniprep again of E.coli cells containing Biobrick parts with Promega, Wizard Plus SV Miniprep DNA Purification System
7/29
Miniprep
- P1.14L(araC)
- P1.7L(lacI)
- P1.4E(cI)
- P1.3D(ColE1)
- P1.9C(p15A)
- P1.9G(p15A)
7/30
Miniprep
- P1.14L(araC)
- P1.7L(lacI)
- P3.21D
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