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From 2009.igem.org

Revision as of 08:52, 16 August 2009 (view source) Unocchi (Talk | contribs) ← Older edit		Revision as of 09:54, 16 August 2009 (view source) Unocchi (Talk | contribs) Newer edit →
Line 1:		Line 1:
-	=parts planning=	+	'''parts planning'''
-		+	=sensor=
	'''signal sensor<br>'''		'''signal sensor<br>'''
	[[Image:Planning_osaka.PNG]]<br>		[[Image:Planning_osaka.PNG]]<br>
-	==1.3O6HSL  P(tetR)+luxI<br>==	+	=='''3O6HSL'''   P(tetR)+luxI<br>==
-	1.1.RBS + luxI<br>	+	1.RBS + luxI<br>
	<table border="1" Frame="box">		<table border="1" Frame="box">
	<tr><td>Vector</td><td>Insert</td></tr>		<tr><td>Vector</td><td>Insert</td></tr>
Line 15:		Line 15:
-	1.2.RBS+ luxI + terminator<br>	+	2.RBS+ luxI + terminator<br>
	<table border="1" Frame="box">		<table border="1" Frame="box">
	<tr><td>Vector</td><td>Insert</td></tr>		<tr><td>Vector</td><td>Insert</td></tr>
	<tr><td>double terminator</td><td>RBS+luxI</td></tr>		<tr><td>double terminator</td><td>RBS+luxI</td></tr>
-	<tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td> </td></tr>	+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
	<tr><td>pSB1AK3</td><td></td></tr>		<tr><td>pSB1AK3</td><td></td></tr>
	</table>		</table>
-	1.3.P(tetR)+RBS+ luxI + terminator<br>	+	3.P(tetR)+RBS+ luxI + terminator<br>
	<table border="1" Frame="box">		<table border="1" Frame="box">
	<tr><td>Vector</td><td>Insert</td></tr>		<tr><td>Vector</td><td>Insert</td></tr>
	<tr><td>BBa_R0040</td><td>RBS+luxI+terminator</td></tr>		<tr><td>BBa_R0040</td><td>RBS+luxI+terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
	<tr><td>pSB1A2</td><td> </td></tr>		<tr><td>pSB1A2</td><td> </td></tr>
	</table>		</table>
-	==2.C4HSL  P(tetR)+rhlI<br>==	+	=='''C4HSL'''   P(tetR)+rhlI<br>==
-	2.1.RBS + rhII	+	1.RBS + rhII
	<table border="1" Frame="box">		<table border="1" Frame="box">
	<tr><td>Vector</td><td>Insert</td></tr>		<tr><td>Vector</td><td>Insert</td></tr>
Line 42:		Line 43:
-	2.2.RBS + rhII + terminator	+	2.RBS + rhII + terminator
	<table border="1" Frame="box">		<table border="1" Frame="box">
	<tr><td>Vector</td><td>Insert</td></tr>		<tr><td>Vector</td><td>Insert</td></tr>
	<tr><td>double terminator</td><td>RBS + rhII </td></tr>		<tr><td>double terminator</td><td>RBS + rhII </td></tr>
-	<tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td> </td></tr>	+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
	<tr><td>pSB1AK3</td><td></td></tr>		<tr><td>pSB1AK3</td><td></td></tr>
	</table>		</table>
-	2.3.P(tetR) + RBS + rhlI + terminator<br>	+	3.P(tetR) + RBS + rhlI + terminator<br>
	<table border="1" Frame="box">		<table border="1" Frame="box">
	<tr><td>Vector</td><td>Insert</td></tr>		<tr><td>Vector</td><td>Insert</td></tr>
Line 60:		Line 61:
-	3.AI-1<br>	+	=='''AI-1'''  P(tetR)+lasI==
-	P(tetR)+lasI<br>	+
		+	1.RBS + LasI
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>RBS</td><td>LasI</td></tr>
		+	<tr><td>pSB1A2</td><td> </td></tr>
		+	</table>
-	4.3OH,C14:1-HSL<br>	+	2.RBS + LasI +terminator
-	P(tetR)+cinI<br>	+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>double terminator</td><td>RBS + LasI +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
		+	<tr><td>pSB1AK3</td><td></td></tr>
		+	</table>
		+
		+	3.P(tetR) + RBS + LasI +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>p(tetR)</td><td>RBS + LasI +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+
		+
		+
		+	=='''3OH,C14:1-HSL'''  P(tetR)+cinI<br>==
		+
		+	1. RBS + cinI
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>RBS</td><td>cinI</td></tr>
		+	<tr><td>pSB1A2</td><td> </td></tr>
		+	</table>
		+
		+
		+	2.RBS + cinI +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>double terminator</td><td>RBS + cinI +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
		+	<tr><td>pSB1AK3</td><td></td></tr>
		+	</table>
		+
		+
		+	3.P(tetR) + RBS + cinI +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>p(tetR)</td><td>RBS + cinI +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+	=receiver=
	'''signal receiver<br>'''		'''signal receiver<br>'''
	[[Image:Planning osaka receiver.PNG]]<br>		[[Image:Planning osaka receiver.PNG]]<br>
-	1.3O6HSL<br>	+	=='''3O6HSL'''  luxpL+luxR+luxpR==
-	luxpL+luxR+luxpR<br>	+	[http://partsregistry.org/Part:BBa_F2621 BBa_F2621](1158bp)<br>
		+	pSB1A2<br>
		+	=='''C4HSL'''  p(tetR) + rhlR  +  RhlR+C4==
		+	1.RhlR+p(Rhl)
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>RhlR</td><td>p(RhlR)</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_I1466 BBa_I1466]</td><td>[http://partsregistry.org/Part:BBa_I14017 BBa_I14017]</td></tr>
		+	<tr><td>pSB2K3</td><td></td></tr>
		+	</table>
-	2.C4HSL<br>	+	2.p(tetR)+RhlR+p(Rhl)
-	p(tetR) + rhlR  +  RhlR+C4<br>	+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>p(tetR)</td><td>RhlR+p(Rhl)</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+	=='''AI-1'''  p(tetR) + lasR +  LasR+pAI==
		+	1. RBS + lasR
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>RBS</td><td>lasR</td></tr>
		+	<tr><td>pSB1A2</td><td> </td></tr>
		+	</table>
-	3.AI-1<br>
-	p(tetR) + lasR +  LasR+pAI
		+	2.RBS + lasR +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>double terminator</td><td>RBS + lasR +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
		+	<tr><td>pSB1AK3</td><td></td></tr>
		+	</table>
-	4.3OH,C14:1-HSL<br>	+	3.P(tetR) + RBS + lasR +terminator
-	p(tetR) + cinR + cinR	+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>p(tetR)</td><td>RBS + lasR +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+
		+
		+	4.P(tetR) + RBS + lasR +terminator +lasR+pAI
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>lasR+pAI</td><td>P(tetR) + RBS + lasR +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]</td><td> </td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+
		+
		+
		+	=='''3OH,C14:1-HSL'''  p(tetR) + cinR + cinR==
		+	1. RBS + cinR
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>RBS</td><td>cinR</td></tr>
		+	<tr><td>pSB1A2</td><td> </td></tr>
		+	</table>
		+
		+
		+	2.RBS + cinR +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>double terminator</td><td>RBS + cinR +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
		+	<tr><td>pSB1AK3</td><td></td></tr>
		+	</table>
		+
		+
		+	3.P(tetR) + RBS + cinR +terminator
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>p(tetR)</td><td>RBS + cinR +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>
		+
		+
		+	4.P(tetR) + RBS + cinR +terminator +p(cinR)
		+	<table border="1" Frame="box">
		+	<tr><td>Vector</td><td>Insert</td></tr>
		+	<tr><td>p(cinR)</td><td>P(tetR) + RBS + cinR +terminator</td></tr>
		+	<tr><td>[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]</td><td> </td></tr>
		+	<tr><td>pSB1A2</td><td></td></tr>
		+	</table>

---

Revision as of 09:54, 16 August 2009

parts planning

Contents 1 sensor 1.1 3O6HSL P(tetR)+luxI 1.2 C4HSL P(tetR)+rhlI 1.3 AI-1 P(tetR)+lasI 1.4 3OH,C14:1-HSL P(tetR)+cinI 2 receiver 2.1 3O6HSL luxpL+luxR+luxpR 2.2 C4HSL p(tetR) + rhlR + RhlR+C4 2.3 AI-1 p(tetR) + lasR + LasR+pAI 2.4 3OH,C14:1-HSL p(tetR) + cinR + cinR

sensor

signal sensor
 

3O6HSL P(tetR)+luxI

1.RBS + luxI

Vector	Insert
RBS	luxI
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]	[http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
pSB1A2

2.RBS+ luxI + terminator

Vector	Insert
double terminator	RBS+luxI
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
pSB1AK3

3.P(tetR)+RBS+ luxI + terminator

Vector	Insert
BBa_R0040	RBS+luxI+terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

C4HSL P(tetR)+rhlI

1.RBS + rhII

Vector	Insert
RBS	rhII
pSB1A2

2.RBS + rhII + terminator

Vector	Insert
double terminator	RBS + rhII
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
pSB1AK3

3.P(tetR) + RBS + rhlI + terminator

Vector	Insert
p(tetR)	RBS + rhII +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

AI-1 P(tetR)+lasI

1.RBS + LasI

Vector	Insert
RBS	LasI
pSB1A2

2.RBS + LasI +terminator

Vector	Insert
double terminator	RBS + LasI +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
pSB1AK3

3.P(tetR) + RBS + LasI +terminator

Vector	Insert
p(tetR)	RBS + LasI +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

3OH,C14:1-HSL P(tetR)+cinI

1. RBS + cinI

Vector	Insert
RBS	cinI
pSB1A2

2.RBS + cinI +terminator

Vector	Insert
double terminator	RBS + cinI +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
pSB1AK3

3.P(tetR) + RBS + cinI +terminator

Vector	Insert
p(tetR)	RBS + cinI +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

receiver

signal receiver
 

3O6HSL luxpL+luxR+luxpR

[http://partsregistry.org/Part:BBa_F2621 BBa_F2621](1158bp)
pSB1A2

C4HSL p(tetR) + rhlR + RhlR+C4

1.RhlR+p(Rhl)

Vector	Insert
RhlR	p(RhlR)
[http://partsregistry.org/Part:BBa_I1466 BBa_I1466]	[http://partsregistry.org/Part:BBa_I14017 BBa_I14017]
pSB2K3

2.p(tetR)+RhlR+p(Rhl)

Vector	Insert
p(tetR)	RhlR+p(Rhl)
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

AI-1 p(tetR) + lasR + LasR+pAI

1. RBS + lasR

Vector	Insert
RBS	lasR
pSB1A2

2.RBS + lasR +terminator

Vector	Insert
double terminator	RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
pSB1AK3

3.P(tetR) + RBS + lasR +terminator

Vector	Insert
p(tetR)	RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

4.P(tetR) + RBS + lasR +terminator +lasR+pAI

Vector	Insert
lasR+pAI	P(tetR) + RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
pSB1A2

3OH,C14:1-HSL p(tetR) + cinR + cinR

1. RBS + cinR

Vector	Insert
RBS	cinR
pSB1A2

2.RBS + cinR +terminator

Vector	Insert
double terminator	RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
pSB1AK3

3.P(tetR) + RBS + cinR +terminator

Vector	Insert
p(tetR)	RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
pSB1A2

4.P(tetR) + RBS + cinR +terminator +p(cinR)

Vector	Insert
p(cinR)	P(tetR) + RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]
pSB1A2

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