August/16 August 2009

From 2009.igem.org

(Difference between revisions)
(→AI-1 P(tetR)+lasI)
(→C4HSL P(tetR)+rhlI)
Line 50: Line 50:
1.RBS + rhII
1.RBS + rhII
<table border="1" Frame="box">
<table border="1" Frame="box">
-
<tr><td>Vector</td><td>Insert</td></tr>
+
<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
<tr><td>RBS</td><td>rhII</td></tr>
<tr><td>RBS</td><td>rhII</td></tr>
<tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td>[http://partsregistry.org/Part:BBa_C0170 BBa_C0170]</td><tr>
<tr><td>[http://partsregistry.org/Part:BBa_B0034 BBa_B0034]</td><td>[http://partsregistry.org/Part:BBa_C0170 BBa_C0170]</td><tr>
Line 60: Line 60:
2.RBS + rhII + terminator
2.RBS + rhII + terminator
<table border="1" Frame="box">
<table border="1" Frame="box">
-
<tr><td>Vector</td><td>Insert</td></tr>
+
<tr><td>Vector(E+X)</td><td>Insert(E+S)</td></tr>
<tr><td>double terminator</td><td>RBS + rhII </td></tr>
<tr><td>double terminator</td><td>RBS + rhII </td></tr>
<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
<tr><td>[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]</td><td> </td></tr>
Line 70: Line 70:
3.P(tetR) + RBS + rhlI + terminator<br>
3.P(tetR) + RBS + rhlI + terminator<br>
<table border="1" Frame="box">
<table border="1" Frame="box">
-
<tr><td>Vector</td><td>Insert</td></tr>
+
<tr><td>Vector(S+P)</td><td>Insert(X+P)</td></tr>
<tr><td>p(tetR)</td><td>RBS + rhII +terminator</td></tr>
<tr><td>p(tetR)</td><td>RBS + rhII +terminator</td></tr>
<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>
<tr><td>[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]</td><td> </td></tr>

Revision as of 11:09, 16 August 2009

parts planning

teble sample

VectorInsert
namename
link to BBa~link to BBa~
total size (bp)insert size (bp)
Plasmid


Contents

sensor

signal sensor
 
Planning osaka.PNG

3O6HSL P(tetR)+luxI

1.RBS + luxI

VectorInsert
RBSluxI
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/Part:BBa_C0161 BBa_C0161]
2091bp585bp
pSB1A2


2.RBS+ luxI + terminator

VectorInsert
double terminatorRBS+luxI
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
4554bp579bp
pSB1AK3


3.P(tetR)+RBS+ luxI + terminator

VectorInsert
BBa_R0040RBS+luxI+terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp726bp
pSB1A2

C4HSL P(tetR)+rhlI

1.RBS + rhII

Vector(S+P)Insert(X+P)
RBSrhII
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/Part:BBa_C0170 BBa_C0170]
2091bp609bp
pSB1A2


2.RBS + rhII + terminator

Vector(E+X)Insert(E+S)
double terminatorRBS + rhII
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
4554bp621bp
pSB1AK3


3.P(tetR) + RBS + rhlI + terminator

Vector(S+P)Insert(X+P)
p(tetR)RBS + rhII +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2625bp750bp
pSB1A2

AI-1 P(tetR)+lasI

1.RBS + LasI

Vector(X+P)Insert(S+P)
RBSLasI
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/wiki/index.php?title=Part:BBa_C0078 BBa_C0078]
2091bp609bp
pSB1A2


2.RBS + LasI +terminator

Vector(E+X)Insert(E+S)
double terminatorRBS + LasI +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
4554bp654bp
pSB1AK3


3.P(tetR) + RBS + LasI +terminator

Vector(S+P)Insert(X+P)
p(tetR)RBS + LasI +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2091bp609bp
pSB1A2

3OH,C14:1-HSL P(tetR)+cinI

1. RBS + cinI

Vector(S+P)Insert(X+P)
RBScinI
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076]
2091bp702bp
pSB1A2


2.RBS + cinI +terminator

Vector(E+X)Insert(E+S)
double terminatorRBS + cinI +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
4554bp714bp
pSB1AK3


3.P(tetR) + RBS + cinI +terminator

Vector(S+P)Insert(X+P)
p(tetR)RBS + cinI +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp843bp
pSB1A2

receiver

signal receiver
 
Planning osaka receiver.PNG


3O6HSL luxpL+luxR+luxpR

[http://partsregistry.org/Part:BBa_F2621 BBa_F2621](1158bp)
pSB1A2


C4HSL p(tetR) + rhlR + RhlR+C4

1.RhlR+p(Rhl)

VectorInsert
RhlRp(RhlR)
[http://partsregistry.org/Part:BBa_I1466 BBa_I1466][http://partsregistry.org/Part:BBa_I14017 BBa_I14017]
4941bp884bp
pSB2K3

2.p(tetR)+RhlR+p(Rhl)

VectorInsert
p(tetR)RhlR+p(Rhl)
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp935bp
pSB1A2

AI-1 p(tetR) + lasR + LasR+pAI

1. RBS + lasR

VectorInsert
RBSlasR
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/wiki/index.php?title=Part:BBa_C0179 BBa_C0179]
2091bp723bp
pSB1A2


2.RBS + lasR +terminator

VectorInsert
double terminatorRBS + lasR +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
4554bp735bp
pSB1AK3


3.P(tetR) + RBS + lasR +terminator

VectorInsert
p(tetR)RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2625bp864bp
pSB1A2


4.P(tetR) + RBS + lasR +terminator +lasR+pAI

VectorInsert
lasR+pAIP(tetR) + RBS + lasR +terminator
[http://partsregistry.org/Part:BBa_R0079 BBa_R0079]
2243bp918bp
pSB1A2

3OH,C14:1-HSL p(tetR) + cinR + cinR

1. RBS + cinR

VectorInsert
RBScinR
[http://partsregistry.org/Part:BBa_B0034 BBa_B0034][http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077]
2091bp762bp
pSB1A2


2.RBS + cinR +terminator

VectorInsert
double terminatorRBS + cinR +terminator
[http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
4554bp774bp
pSB1AK3


3.P(tetR) + RBS + cinR +terminator

VectorInsert
p(tetR)RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]
2133bp903bp
pSB1A2


4.P(tetR) + RBS + cinR +terminator +p(cinR)

VectorInsert
p(cinR)P(tetR) + RBS + cinR +terminator
[http://partsregistry.org/Part:BBa_R0078 BBa_R0078]
2304bp957bp
pSB1A2
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