Team:UQ-Australia/Notebook

From 2009.igem.org

(Difference between revisions)
(→Water Purification Project)
(→Bioprecipitation Project)
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=='''Bioprecipitation Project'''==
=='''Bioprecipitation Project'''==
-
'''5/08/09'''
+
'''11/08/09'''
 +
No results were given from the transformation. Transformation will have to be repeated.
-
Plasmids have arrived!
 
-
*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-E/J pXCK-E/J]
 
-
*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-K pXCK-K]
 
-
*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-EL pXCK-EL]
 
-
*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-ES pXCK-ES]
 
-
We have put them into 150 mL of TE buffer and left overnight for the paper to dissolve and later extract the plasmid from the solution.
+
'''10/08/09'''
 +
Transformation of our 4 plasmids took place. They were transformed into TOP10 bacteria. A colony check will be preformed tomorrow morning.  
-
A Nanodrop was used to see if the DNA was coming out of the paper. No DNA was detected, thus samples were left overnight.
+
To see protocol, click [https://2009.igem.org/Team:UQ-Australia/Notebook/Transformation_procedure HERE]
-
''Click on the plasmid name to look at the vector''
 
 +
'''7/08/09'''
 +
Vectors were examined and we are planning for the best cloning strategy for the standard plasmids.
'''6/08/09'''
'''6/08/09'''
-
 
Plasmid solutions were left overnight and in the morning checked again with Nanodrop. No DNA was measured. Samples were put in the waterbath at 37 degrees and left for a couple of hours. If Nanodrop does not work after the waterbath we will still do transformation since we only need few plasmids for the transformation to work. Nanodrop may not have been able to quantify such a small concentration of plasmids.
Plasmid solutions were left overnight and in the morning checked again with Nanodrop. No DNA was measured. Samples were put in the waterbath at 37 degrees and left for a couple of hours. If Nanodrop does not work after the waterbath we will still do transformation since we only need few plasmids for the transformation to work. Nanodrop may not have been able to quantify such a small concentration of plasmids.
Line 468: Line 465:
Plasmids were stored and transformation will be done on Monday.
Plasmids were stored and transformation will be done on Monday.
-
'''7/08/09'''
 
-
Vectors were examined and we are planning for the best cloning strategy for the standard plasmids.
 
 +
'''5/08/09'''
-
'''10/08/09'''
+
Plasmids have arrived!
-
Transformation of our 4 plasmids took place. They were transformed into TOP10 bacteria. A colony check will be preformed tomorrow morning.  
+
*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-E/J pXCK-E/J]
 +
*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-K pXCK-K]
 +
*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-EL pXCK-EL]
 +
*[https://2009.igem.org/Team:UQ-Australia/Notebook/pXCK-ES pXCK-ES]
-
To see protocol, click [https://2009.igem.org/Team:UQ-Australia/Notebook/Transformation_procedure HERE]
+
We have put them into 150 mL of TE buffer and left overnight for the paper to dissolve and later extract the plasmid from the solution.
-
'''11/08/09'''
+
A Nanodrop was used to see if the DNA was coming out of the paper. No DNA was detected, thus samples were left overnight.
-
No results were given from the transformation. Transformation will have to be repeated.
+
 
 +
''Click on the plasmid name to look at the vector''

Revision as of 14:39, 16 August 2009

Water Purification Project

24/07
Mini prep DNA extractions performed for MS427-pBAD, MS427-pKKJ143 cultures incubated overnight.
--> run on ethidium bromide gel to confirm DNA production.

23/07/09
E. coli incubated overnight in duplicate under the following conditions:

Strain: MS427 

Plasmid: pBAD (empty plasmid -AG43) 

- 2mL LB broth 

- 2µL ampicillin 




Strain: MS427 

Plasmid: pKKJ143 (plasmid w/ AG43) 

- 2mL LB broth 

- 2µL ampicillin

17/07/09 - MG1655 Stock Preparation
MG1655 E. coli grown on pure LB stock. No growth was observed using LB + ampicillin medium.
Pelleted and resuspended in 50:50 glycerol:LB medium and stored at -80 degrees C.

Bioprecipitation Project

11/08/09 No results were given from the transformation. Transformation will have to be repeated.


10/08/09 Transformation of our 4 plasmids took place. They were transformed into TOP10 bacteria. A colony check will be preformed tomorrow morning.

To see protocol, click HERE


7/08/09 Vectors were examined and we are planning for the best cloning strategy for the standard plasmids.


6/08/09 Plasmid solutions were left overnight and in the morning checked again with Nanodrop. No DNA was measured. Samples were put in the waterbath at 37 degrees and left for a couple of hours. If Nanodrop does not work after the waterbath we will still do transformation since we only need few plasmids for the transformation to work. Nanodrop may not have been able to quantify such a small concentration of plasmids.

LB media was poured on petri dishes and plasmid DNA was checked again using Nanodrop.

  • pXCK-EL = 10.55 ng/uL
  • pXCK-ES = 8.43 ng/uL
  • pXCK-K = 11.73 ng/uL
  • pXCK-E/J = 2.66 ng/uL

Plasmids were stored and transformation will be done on Monday.


5/08/09

Plasmids have arrived!

We have put them into 150 mL of TE buffer and left overnight for the paper to dissolve and later extract the plasmid from the solution.

A Nanodrop was used to see if the DNA was coming out of the paper. No DNA was detected, thus samples were left overnight.

Click on the plasmid name to look at the vector

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