Team:Paris/Protocols
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===4. Molecular biology=== | ===4. Molecular biology=== | ||
*[[Team:Paris/ProtocolsMB#PCR with Quick load Taq2x Master Mix|PCR]] | *[[Team:Paris/ProtocolsMB#PCR with Quick load Taq2x Master Mix|PCR]] | ||
+ | *[[Team:Paris/ProtocolsMB#PCR colonies|PCR colony]] | ||
* Gels | * Gels | ||
* Digestion | * Digestion | ||
* Ligation | * Ligation |
Revision as of 22:39, 16 August 2009
Contents |
Protocols
Here you will find the collection of protocole we use, or just collect. And because we are well-known chauvinist it is just a tribute to previous Paris iGEM team . We try our best not to make it redondant.
Summary
1. Microscopy
- Protocols suggested
- [http://probes.invitrogen.com/media/pis/mp00282.pdf Protocol to membrane dying, DID method (pdf)]
2. Adapted Protocols
- DNA extraction
- Mini prep
- Gel/PCR Purification
3. Culture protocols
4. Molecular biology
- PCR
- PCR colony
- Gels
- Digestion
- Ligation