Template:Team:KULeuven/19 August 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
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* Miniprep Sam8, Sam5, Ech, Fcs plasmids in triple
* Miniprep Sam8, Sam5, Ech, Fcs plasmids in triple
-
--> nanodrop concentrations:
+
* Nanodrop concentrations:
 +
{| border ="1" align="center"
 +
!| Part || concentration (ng/μl) || 260/280 λ
 +
|- align="center"
 +
| Sam8 A ||58,2 || 1,87 ||
 +
|- align="center"
 +
| Sam8 B ||63,2 || 1,93 ||
 +
|- align="center"
 +
| Sam5 A ||94,6 || 1,92 ||
 +
|- align="center"
 +
| Sam5 B ||125,0 || 1,91 ||
 +
|- align="center"
 +
| Sam5 C ||104,2 || 1,91 ||
 +
|- align="center"
 +
| Ech A ||88,6 || 1,92 ||
 +
|- align="center"
 +
| Ech B ||96,7 || 1,95 ||
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|- align="center"
 +
| Ech C ||105,6 || 1,86 ||
 +
|- align="center"
 +
| Fcs A ||84,4 || 1,88 ||
 +
|- align="center"
 +
| Fcs B ||113,4 || 1,95 ||
 +
|- align="center"
 +
| Fcs C ||90,3 || 1,92 ||
 +
|-
 +
|}

Revision as of 09:34, 20 August 2009

  • Miniprep Sam8, Sam5, Ech, Fcs plasmids in triple
  • Nanodrop concentrations:
Part concentration (ng/μl) 260/280 λ
Sam8 A 58,2 1,87
Sam8 B 63,2 1,93
Sam5 A 94,6 1,92
Sam5 B 125,0 1,91
Sam5 C 104,2 1,91
Ech A 88,6 1,92
Ech B 96,7 1,95
Ech C 105,6 1,86
Fcs A 84,4 1,88
Fcs B 113,4 1,95
Fcs C 90,3 1,92


  • We took the plasmids with the lowest concentrations to perform the restriction test, described above. Afterwards, we ran the restriction products, together with uncut plasmids, on a gel.
  • Meanwhile ... one plated EF colony seemed to have grown. Made fluid culture of EF.
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