Team:KULeuven/Freezer

From 2009.igem.org

(Difference between revisions)
(→vanillin synthesis)
(→vanillin receptor)
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= vanillin receptor =
= vanillin receptor =
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{| class="Generic sortable"
 
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! class='top' | part
 
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! class='top' | plasmid
 
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! class='top' | description
 
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! class='top' | status
 
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! class='top' | μl left
 
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! class='top' | concentration
 
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! class='top' | date
 
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|-
 
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| 2235 || / ||forward primer for {{kulpart|BBa_K238008}} || || || 20μM || August 3
 
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|-
 
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| 2238 || / || reverse primer for {{kulpart|BBa_K238008}} || || || 20μM || August 3
 
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|-
 
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| 2235 || / || forward primer for {{kulpart|BBa_K238008}} || || || 100μM || August 3
 
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|-
 
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| 2238 || / || reverse primer for {{kulpart|BBa_K238008}} || || || 100μM || August 3
 
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|-
 
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| 2242 || / || forward primer for {{kulpart|BBa_K238009}} || || || 20μM ||August 3
 
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|-
 
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| 2243 || / || reverse primer for {{kulpart|BBa_K238009}} || || || 20μM || August 3
 
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|-
 
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| 2242 || / || forward primer for {{kulpart|BBa_K238009}} || || || 100μM || August 3
 
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|-
 
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| 2243 || / || reverse primer for {{kulpart|BBa_K238009}} || || || 100μM || August 3
 
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|-
 
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| 2244 || / ||forward primer for {{kulpart|BBa_K238010}} || || || 20μM || August 3
 
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|-
 
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| 2245 || / || reverse primer for {{kulpart|BBa_K238010}} || || || 20μM || August 3
 
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|-
 
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| 2244 || / || forward primer for {{kulpart|BBa_K238010}} || || || 100μM || August 3
 
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|-
 
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| 2245 || / || reverse primer for {{kulpart|BBa_K238010}} || || || 100μM || August 3
 
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|-
 
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| 2246 || / || forward primer for {{kulpart|BBa_K238011}} || || || 20μM ||August 3
 
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|-
 
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| 2247 || / || reverse primer for {{kulpart|BBa_K238011}} || || || 20μM || August 3
 
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|-
 
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| 2246 || / || forward primer for {{kulpart|BBa_K238011}} || || || 100μM || August 3
 
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|-
 
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| 2247 || / || reverse primer for {{kulpart|BBa_K238011}} || || || 100μM || August 3
 
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|-
 
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| 2288 || / || forward primer for mutagenesis for {{kulpart|BBa_K238008}} || || || 20μM || August 8
 
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|-
 
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| 2289 || / || reverse primer for mutagenesis for {{kulpart|BBa_K238008}} || || || 20μM || August 8
 
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|-
 
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| 2288 || / || forward primer for mutagenesis for {{kulpart|BBa_K238008}} || || || 100μM || August 8
 
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|-
 
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| 2289 || / || reverse primer for mutagenesis for {{kulpart|BBa_K238008}} || || || 100μM || August 8
 
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|-
 
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| 2290 || / || forward primer for mutagenesis for {{kulpart|BBa_K238008}} || || || 20μM || August 8
 
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|-
 
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| 2291 || / || reverse primer for mutagenesis for {{kulpart|BBa_K238008}} || || || 20μM || August 8
 
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|-
 
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| 2290 || / || forward primer for mutagenesis for {{kulpart|BBa_K238008}} || || || 100μM || August 8
 
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|-
 
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| 2291 || / || reverse primer for mutagenesis for {{kulpart|BBa_K238008}} || || || 100μM || August 8
 
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|-
 
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| 2292 || / || forward primer for mutagenesis for {{kulpart|BBa_K238010}} || || || 20μM || August 8
 
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|-
 
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| 2293 || / || reverse primer for mutagenesis for {{kulpart|BBa_K238010}} || || || 20μM || August 8
 
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|-
 
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| 2292 || / || forward primer for mutagenesis for {{kulpart|BBa_K238010}} || || || 100μM || August 8
 
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|-
 
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| 2293 || / || reverse primer for mutagenesis for {{kulpart|BBa_K238010}} || || || 100μM || August 8
 
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|-
 
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| 2294 || / || forward primer for mutagenesis for {{kulpart|BBa_K238010}} || || || 20μM || August 8
 
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|-
 
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| 2295 || / || reverse primer for mutagenesis for {{kulpart|BBa_K238010}} || || || 20μM || August 8
 
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|-
 
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| 2294 || / || forward primer for mutagenesis for {{kulpart|BBa_K238010}} || || || 100μM || August 8
 
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|-
 
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| 2295 || / || reverse primer for mutagenesis for {{kulpart|BBa_K238010}} || || || 100μM || August 8
 
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|-}
 
= miscellaneous =
= miscellaneous =

Revision as of 12:03, 20 August 2009

Contents

Blue light receptor

part plasmid description status μl left concentration date
MC4100 / strain of E.coli july 24
2171 / forward primer for 90µl 20μM july 24
2172 / reverse primer for 90µl 20μM july 24
2171 / forward primer for 100μM july 24
2172 / reverse primer for 100μM july 24
2260 / forward primer for 45µl 20μM aug 10
2261 / reverse primer for 45µl 20μM aug 10
2260 / forward primer for 100μM aug 10
2261 / reverse primer for 100μM aug 10
pSB1A2GFP + rbs + terminatorrestricted with EcoRI and XbaIjuly 29
pSB1A2GFP + LVA-tag + terminatorrestricted with EcoRI and XbaIjuly 29
/BLR promoter region (1:200 adn 1:500)restricted with EcoRIjuly 29
/PCR BLR promoter region (360bp)4 microcentrifuge tubesjuly 30
pSB3K3 kanamycine plasmide backbone taken from plates 13μl aug 11
/Exact blue light promotor pcr product / 123,3 ng/μl aug 13
/constitutive promotortaken from plates 13μl aug 11
/Exact blue light promotor cut with EcoRI and SpeI 26μl 27 ng/μl aug 12
/LigA: Exact blue light promotor ligated with E0240 in pSB1A2 T4 DNA ligation 18μl aug 13
/Lig B: Exact blue light promotor ligated with K145015 in pSB1A2 T4 DNA ligation 18μl aug 14
/Lig A: Exact blue light promotor ligated with E02040 in pSB1A2 (1,3,5,7,9) cut with EcoRI and PstI 5 x 18μl aug 18

Key/antikey/lock

part plasmid description status μl left concentration date
/GFP with LVA tagpurifiedjuly 28
/tetR promoter purifiedjuly 28

vanillin synthesis

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid (oranje)108,8 ng/µlAugust 4
pSB1A2fcs + rbspurified plasmid (oranje)113,9 ng/µlAugust 4
pSB1A2Sam5 + rbsreceived from EdinburghLost in transitAugust 3
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid (oranje)101,3 ng/µlAugust 5
pSB1A2Sam8 + rbspurified plasmid (oranje)95,7 ng/µlAugust 5
pSB1A2Sam8 (b)purified plasmid (oranje)81,5 ng/µlAugust 5
pSB1A2COMTpurified plasmid (oranje)122,9 ng/µlAugust 5
pSB1A2ech + rbspurified digest (C2)16,9 ng/µlAugust 6
fcs + rbspurified digest (C1, FCS Rb)16,6 ng/µlAugust 6
pSB1A2sam5 + rbspurified digest (A2)3,1 ng/µlAugust 7
sam8 + rbspurified digest (A1)3,4 ng/µl August 7
pSB1A2sam5 + sam8ligation (lig. A)August 11
pSB1A2ech + fcsligation (lig.C)August 11
pSB1A2fcs + rbspurified plasmid (fcs pl.)29 µl57,2 ng/µlAugust 12
pSB1A2Sam5 + rbspurified plasmid (sam5 pl.)31 µl76,0 ng/µlAugust 12
fcs + rbspurified digest (F rd.)18 µl4,2 ng/µlAugust 12
pSB1A2sam5 + rbspurified digest (5 rd.)18 µl7,3 ng/µlAugust 12
pSB1A2ech + rbspurified plasmid (ech pl.)38 µl72,7 ng/µlAugust 13
pSB1A2Sam8 + rbspurified plasmid (sam8 pl.)38 µl66,2 ng/µlAugust 13
sam8 + rbspurified digest (sam8 rd.)16 µl9,4 ng/µlAugust 14
pSB1A2ech + rbspurified digest (ech rd.)16 µl13,6 ng/µlAugust 14
pSB1A2sam8 + Sam5ligation (SAMS)August 14
pSB1A2ech + fcsligation (EF)August 14
pSB1A2ech + rbspurified plasmid (ech A)36,8 µl88,6 ng/µlAugust 19
pSB1A2ech + rbspurified plasmid (ech B)48 µl96,7 ng/µlAugust 19
pSB1A2ech + rbspurified plasmid (ech C)48 µl105,6 ng/µlAugust 19
pSB1A2fcs + rbspurified plasmid (fcs A)30,3 µl84,8 ng/µlAugust 19
pSB1A2fcs + rbspurified plasmid (fcs B)48 µl113,4 ng/µlAugust 19
pSB1A2fcs + rbspurified plasmid (fcs C)48 µl90,3 ng/µlAugust 19
pSB1A2Sam5 + rbspurified plasmid (sam5 A)37,4 µl94,6 ng/µlAugust 19
pSB1A2Sam5 + rbspurified plasmid (sam5 B)48 µl125,0 ng/µlAugust 19
pSB1A2Sam5 + rbspurified plasmid (sam5 C)48 µl104,2 ng/µlAugust 19
pSB1A2Sam8 + rbspurified plasmid (sam8 A)21,9 µl58,2 ng/µlAugust 19
pSB1A2Sam8 + rbspurified plasmid (sam8 B)48 µl63,2 ng/µlAugust 19

vanillin receptor

miscellaneous

part plasmid description status μl left concentration date
dNTP mixATP, GTP, TTP, CTP9410μMaug 10
pSB1A2medium constitutieve promoterjuly 23
ampicilline
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