From 2009.igem.org
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Revision as of 05:12, 21 August 2009
University of Calgary
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CAROL
Descriptive Title of What You’re Doing
WIKI CODING HERE
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CHINMOYEE
Three Gene Repressilator Presentation
Presented Ander's Modeling examples of the Three Gene Repressilator .
Looked at the Optimization Toolbox:
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EMILY
Friday Team Meeting
- Had a team meeting where each subproject presented their recent work. Lab group talked about verification of genes of interest in TOPO vectors, BBK ampliciation PCR and cloning into Biobrick vectors (psB1AC3 and psB1AK3).
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FAHD
Descriptive Title of What You're Doing
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IMAN
Descriptive Title of What You're Doing
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JAMIE
Descriptive Title of What You're Doing
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JEREMY
Descriptive Title of What You're Doing
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KATIE
Descriptive Title of What You're Doing
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KEVIN
Team meeting
No lab experiments were performed
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MANDY
Descriptive Title of What You're Doing
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PATRICK
Descriptive Title of What You're Doing
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PRIMA
Descriptive Title of What You're Doing
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STEFAN
Descriptive Title of What You're Doing
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VICKI
Purification of gradient PCR product
Purpose: to purify the LuxOD47A BBk that was produced yesterday in the gradient PCR
Protocol: Please refer to the protocol page. Concentrations were measured after the product was purified.
Restriction digest of LuxOD47A BBk (insert) and psB1AC3 (vector)
Purpose: The LuxOD47A BBk DNA from yesterday’s gradient PCR is in linear form. This will prepare the ends so that it can be inserted into a BioBrick vector.
Protocol:
This is the restriction digest component of the construction protocol, which we describe on our protocol page. We attempted the construction with 2 different sets of enzymes: 6 tubes with cuts at XbaI and PstI , and 6 tubes with cuts at EcoRI and PstI. Both were prepared in REact 2 buffer. Once prepared, the tubes were left in the water bath at 37 degrees C for 6.5 hours; heatshocked at 65 degrees C on a heating block to deactivate the restriction enzymes in the tube; and placed in the -20 degrees C freezer.
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