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Template:Team:KULeuven/21 August 2009/VanillinProduction
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(New page: * Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly. However, the EF results were not exspected. It seems that somehow the bacteria had ...)
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(New page: * Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly. However, the EF results were not exspected. It seems that somehow the bacteria had ...)
Newer edit →
Revision as of 13:01, 21 August 2009
- Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly.
However, the EF results were not exspected. It seems that somehow the bacteria had taken in a self-closed Ech-plasmid. The bands do not correspond with an EF-ligation product.
- The DNA was purified from the gel
Nanodrop results:
| Part | ng/µl | 260/280 | |
|---|---|---|---|
| Sam8 | 2,5 | 1,14 | |
| Sam5 | 7,9 | 1,60 | |
| Ech | 5,0 | 1,38 | |
| Fcs | 3,4 | 0,93 |
- Sam8 and Sam5; Fcs and Ech were ligated
Used volumes:
| Part | µl | |
|---|---|---|
| Sam8 | 34,0 | |
| Sam5 | 6,3 | |
| Ech | 10,0 | |
| Fcs | 35,8 |
