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Template:Team:KULeuven/21 August 2009/VanillinProduction
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(New page: * Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly. However, the EF results were not exspected. It seems that somehow the bacteria had ...) |
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| - | * Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly. | + | * '''Test 2''': We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly. |
However, the EF results were not exspected. It seems that somehow the bacteria had taken in a self-closed Ech-plasmid. The bands do not correspond with an EF-ligation product. | However, the EF results were not exspected. It seems that somehow the bacteria had taken in a self-closed Ech-plasmid. The bands do not correspond with an EF-ligation product. | ||
Revision as of 13:02, 21 August 2009
- Test 2: We loaded yesterday's restrictions on an agarose gel. It showed that the enzymes cut properly.
However, the EF results were not exspected. It seems that somehow the bacteria had taken in a self-closed Ech-plasmid. The bands do not correspond with an EF-ligation product.
- The DNA was purified from the gel
Nanodrop results:
| Part | ng/µl | 260/280 | |
|---|---|---|---|
| Sam8 | 2,5 | 1,14 | |
| Sam5 | 7,9 | 1,60 | |
| Ech | 5,0 | 1,38 | |
| Fcs | 3,4 | 0,93 |
- Sam8 and Sam5; Fcs and Ech were ligated
Used volumes:
| Part | µl | |
|---|---|---|
| Sam8 | 34,0 | |
| Sam5 | 6,3 | |
| Ech | 10,0 | |
| Fcs | 35,8 |
