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Team:Virginia/3 June 2009
From 2009.igem.org
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**JW3470-1 (arsC knockout) | **JW3470-1 (arsC knockout) | ||
| + | *Set up dilutions of DH5a E.coli to get OD v cell count calibration | ||
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|+Cell Count of Serial 1:10 dilutions | |+Cell Count of Serial 1:10 dilutions | ||
Revision as of 19:57, 3 June 2009
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Overnight Results
Results of plating
- psB1T3 Nothing
- psB1C3 Small colonies
- psB4A5 lawn
- psB1A3 lawn
- psB1K3 Small colonies
Today
- Mini-Prep of Factor 7.
- Repeat of growth curve standards.
- k12
- DBH5-alpha
- Glycerol stocks (15%) for -80 freezer of
- JW3469-1 (arsB knockout)
- JW3470-1 (arsC knockout)
- Set up dilutions of DH5a E.coli to get OD v cell count calibration
| Plate Num. | Optical Density | Original Concentration (X unknown) | 1:10^1 | 1:10^2 | 1:10^3 | 1:10^4 | 1:10^5 | 1:10^6 | 1:10^7 | 1:10^8 |
| 1 | 0.57 | X | ||||||||
| 2 | 0.41 | .75X | ||||||||
| 3 | 0.27 | .5X | ||||||||
| 4 | 0.1 | .25X | ||||||||
| 5 | 0 | .1X |
