August/21 August 2009

From 2009.igem.org

(Difference between revisions)
(→transformation)
(→check the result of transformation)
Line 13: Line 13:
  2-13N  <15
  2-13N  <15
  ⑤　　　　　　0
  ⑤　　　　　　0
 +
 +
===restriction and digestion===
 +
 +
ⅰ　パーツ⑨の作成
 +
　Insert
 +
  H2O　　　　    ４
 +
　No.2　buffer 2
 +
①ー３　      12
 +
EcoRI

Revision as of 00:42, 22 August 2009

transformation

to get new plasmid

⑤(RBS+rhlR+terminator+promoter(reg))
⑦（RBS＋cinR)
⑧（RBS＋luxI)

check the result of transformation

2-8G     4
2-21F    0    
2-6O     2
2-13N   <15
⑤　　　　　　0

restriction and digestion

ⅰ　パーツ⑨の作成 　Insert 

 H2O　　　　    ４

　No.2　buffer 2 

①ー３　       12
EcoRI
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