August/21 August 2009

From 2009.igem.org

(Difference between revisions)
(restriction and digestion)
(restriction and digestion)
Line 22: Line 22:
①ー3        12
①ー3        12
EcoRI      1
EcoRI      1
-
SpeⅠ     
+
SpeⅠ            

Revision as of 00:45, 22 August 2009

transformation

to get new plasmid

⑤(RBS+rhlR+terminator+promoter(reg))
⑦(RBS+cinR)
⑧(RBS+luxI)

check the result of transformation

2-8G     4
2-21F    0    
2-6O     2
2-13N   <15
⑤      0

restriction and digestion

ⅰ パーツ⑨ Insert H2O      4 No.2 buffer  2 ①ー3   12 EcoRI   1 SpeⅠ