Modelling

From 2009.igem.org

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Revision as of 20:57, 3 June 2009

To find

PDB files of LOV domain, TrpR repressor => [http://www.rcsb.org/pdb/home/home.do PDB database]

To do

OK - Set up day of starting modeling, do a planning for modeling => see with Matteo Dal Peraro ----> will be setted up as soon as the exams are over

Ask Matteo to send all the required starting papers, files, and links to tutorials BEFORE the end of the exams (around midjune)

- Model allosteric interactions between LOVTAP & TrpR:

What will be done:
-> Model of LOVTAP in dark phase
-> Model of LOVTAP in light phase
-> Characterize how the J-alpha helix changes
-> Model sturctural changes that enhance the switch feature of LOVTAP e.g. in dark phase: really weak interaction between LOVTAP and the corresponding DNA sequence, in light phase: strong binding of LOVTAP on DNA.

We will follow the following article protocol:
Freddolino, P.L., Dittrich M., Schulten K., Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins. Biophysical Journal, 91, 3630-3639, 2006 ([http://www.ncbi.nlm.nih.gov/sites/entrez?db=pmc&cmd=search&term=PMC1630464 Pubmed])

- Model the fusion of LOV domain with other transcription factors </b>

To envisage

- Molecular mutationnal assay

Home The Team The Project Parts Submitted to the Registry Modeling Notebook Lectures Team Management

@@ Line 18: / Line 18: @@
 <br> Freddolino, P.L., Dittrich M., Schulten K., Dynamic Switching Mechanisms in LOV1 and LOV2 Domains of Plant Phototropins. Biophysical Journal, 91, 3630-3639, 2006 ([http://www.ncbi.nlm.nih.gov/sites/entrez?db=pmc&cmd=search&term=PMC1630464 Pubmed])
-<br>- Model the fusion of LOV domain with other transcription factors </b>
+<br>- Model the fusion of LOV domain with other transcription factors
+</b>
 ==To envisage ==