Team:KULeuven/Freezer

From 2009.igem.org

(Difference between revisions)
(→vanillin synthesis)
(→vanillin synthesis)
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|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (oranje)||||108,8 ng/µl||August 4
|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (oranje)||||108,8 ng/µl||August 4
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|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (oranje)||||113,9 ng/µl||August 4
 
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|{{kulpart|BBa_I742105}}||pSB1A2||Sam5 + rbs||received from Edinburgh||Lost in transit||||August 3
 
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|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid (oranje)||||101,3 ng/µl||August 5
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs, PstI site removed||purified plasmid (oranje)||||101,3 ng/µl||August 5
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|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (oranje)||||95,7 ng/µl||August 5
 
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|{{kulpart|BBa_I742142}}||pSB1A2||Sam8 (b)||purified plasmid (oranje)||||81,5 ng/µl||August 5
 
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|{{kulpart|BBa_I742107}}||pSB1A2||COMT||purified plasmid (oranje)||||122,9 ng/µl||August 5
|{{kulpart|BBa_I742107}}||pSB1A2||COMT||purified plasmid (oranje)||||122,9 ng/µl||August 5
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|||pSB1A2||ech + rbs||purified digest (C2)||||16,9 ng/µl||August 6
 
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|||||fcs + rbs||purified digest (C1, FCS Rb)||||16,6 ng/µl||August 6
 
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|||pSB1A2||sam5 + rbs||purified digest (A2)||||3,1 ng/µl||August 7
 
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|||||sam8 + rbs||purified digest (A1)||||3,4 ng/µl ||August 7
 
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|||pSB1A2||sam5 + sam8||ligation (lig. A)||||||August 11
 
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|||pSB1A2||ech + fcs||ligation (lig.C)||||||August 11
 
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|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs pl.)||29 µl||57,2 ng/µl||August 12
|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs pl.)||29 µl||57,2 ng/µl||August 12
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|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 pl.)||31 µl||76,0 ng/µl||August 12
|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 pl.)||31 µl||76,0 ng/µl||August 12
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|||||fcs + rbs||purified digest (F rd.)||18 µl||4,2 ng/µl||August 12
 
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|||pSB1A2||sam5 + rbs||purified digest (5 rd.)||18 µl||7,3 ng/µl||August 12
 
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|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech pl.)||38 µl||72,7 ng/µl||August 13
 
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|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 pl.)||38 µl||66,2 ng/µl||August 13
 
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|||||sam8 + rbs||purified digest (sam8 rd.)||16 µl||9,4 ng/µl||August 14
 
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|||pSB1A2||ech + rbs||purified digest (ech rd.)||16 µl||13,6 ng/µl||August 14
 
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|||pSB1A2||sam8 + Sam5||ligation (SAMS)||||||August 14
 
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|||pSB1A2||ech + fcs||ligation (EF)||||||August 14
 
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|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech A)||0 µl||88,6 ng/µl||August 19
|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech A)||0 µl||88,6 ng/µl||August 19
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|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 B)||40,1 µl||63,2 ng/µl||August 19
|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 B)||40,1 µl||63,2 ng/µl||August 19
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|||pSB1A2||sam8 + sam5||ligation (SAMS)||||||August 24
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|||pSB1A2||sam8 + sam5||ligation (SAMS 21)||||||August 24
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|||pSB1A2||fcs + ech||ligation (EF)||||||August 24
+
|||pSB1A2||fcs + ech||ligation (EF 21)||||||August 24
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Revision as of 09:05, 26 August 2009

Contents

Blue light receptor

part plasmid description status μl left concentration date
MC4100 / strain of E.coli july 24
2171 / forward primer for 90µl 20μM july 24
2172 / reverse primer for 90µl 20μM july 24
2171 / forward primer for 100μM july 24
2172 / reverse primer for 100μM july 24
2260 / forward primer for 45µl 20μM aug 10
2261 / reverse primer for 45µl 20μM aug 10
2260 / forward primer for 100μM aug 10
2261 / reverse primer for 100μM aug 10
pSB1A2GFP + rbs + terminatorrestricted with EcoRI and XbaIjuly 29
pSB1A2GFP + LVA-tag + terminatorrestricted with EcoRI and XbaIjuly 29
/BLR promoter region (1:200 adn 1:500)restricted with EcoRIjuly 29
/PCR BLR promoter region (360bp)4 microcentrifuge tubesjuly 30
pSB3K3 kanamycine plasmide backbone taken from plates 13μl aug 11
/Exact blue light promotor pcr product / 123,3 ng/μl aug 13
/constitutive promotortaken from plates 13μl aug 11
/Exact blue light promotor cut with EcoRI and SpeI 26μl 27 ng/μl aug 12
/LigA: Exact blue light promotor ligated with E0240 in pSB1A2 T4 DNA ligation 18μl aug 13
/Lig B: Exact blue light promotor ligated with K145015 in pSB1A2 T4 DNA ligation 18μl aug 14
/Lig A: Exact blue light promotor ligated with E02040 in pSB1A2 (1,3,5,7,9) cut with EcoRI and PstI 5 x 18μl aug 18

Key/antikey/lock

part plasmid description status μl left concentration date
/GFP with LVA tagpurifiedjuly 28
/tetR promoter purifiedjuly 28

vanillin synthesis

part plasmid description status μl left concentration date
pSB1A2ech + rbspurified plasmid (oranje)108,8 ng/µlAugust 4
pSB1A2Sam5 + rbs, PstI site removedpurified plasmid (oranje)101,3 ng/µlAugust 5
pSB1A2COMTpurified plasmid (oranje)122,9 ng/µlAugust 5
pSB1A2fcs + rbspurified plasmid (fcs pl.)29 µl57,2 ng/µlAugust 12
pSB1A2Sam5 + rbspurified plasmid (sam5 pl.)31 µl76,0 ng/µlAugust 12
pSB1A2ech + rbspurified plasmid (ech A)0 µl88,6 ng/µlAugust 19
pSB1A2ech + rbspurified plasmid (ech B)42,8 µl96,7 ng/µlAugust 19
pSB1A2ech + rbspurified plasmid (ech C)48 µl105,6 ng/µlAugust 19
pSB1A2fcs + rbspurified plasmid (fcs A)0 µl84,8 ng/µlAugust 19
pSB1A2fcs + rbspurified plasmid (fcs B)43,6 µl113,4 ng/µlAugust 19
pSB1A2fcs + rbspurified plasmid (fcs C)48 µl90,3 ng/µlAugust 19
pSB1A2Sam5 + rbspurified plasmid (sam5 A)0 µl94,6 ng/µlAugust 19
pSB1A2Sam5 + rbspurified plasmid (sam5 B)44 µl125,0 ng/µlAugust 19
pSB1A2Sam5 + rbspurified plasmid (sam5 C)48 µl104,2 ng/µlAugust 19
pSB1A2Sam8 + rbspurified plasmid (sam8 A)0 µl58,2 ng/µlAugust 19
pSB1A2Sam8 + rbspurified plasmid (sam8 B)40,1 µl63,2 ng/µlAugust 19
pSB1A2sam8 + sam5ligation (SAMS 21)August 24
pSB1A2fcs + echligation (EF 21)August 24

vanillin receptor

part plasmid description status μl left concentration date
2235 / forward primer for 20μM August 3
2238 / reverse primer for 20μM August 3
2235 / forward primer for 100μM August 3
2238 / reverse primer for 100μM August 3
2242 / forward primer for 20μM August 3
2243 / reverse primer for 20μM August 3
2242 / forward primer for 100μM August 3
2243 / reverse primer for 100μM August 3
2244 / forward primer for 20μM August 3
2245 / reverse primer for 20μM August 3
2244 / forward primer for 100μM August 3
2245 / reverse primer for 100μM August 3
2246 / forward primer for 20μM August 3
2247 / reverse primer for 20μM August 3
2246 / forward primer for 100μM August 3
2247 / reverse primer for 100μM August 3
2288 / forward primer for mutagenesis for 20μM August 8
2289 / reverse primer for mutagenesis for 20μM August 8
2288 / forward primer for mutagenesis for 100μM August 8
2289 / reverse primer for mutagenesis for 100μM August 8
2290 / forward primer for mutagenesis for 20μM August 8
2291 / reverse primer for mutagenesis for 20μM August 8
2290 / forward primer for mutagenesis for 100μM August 8
2291 / reverse primer for mutagenesis for 100μM August 8
2292 / forward primer for mutagenesis for 20μM August 8
2293 / reverse primer for mutagenesis for 20μM August 8
2292 / forward primer for mutagenesis for 100μM August 8
2293 / reverse primer for mutagenesis for 100μM August 8
2294 / forward primer for mutagenesis for 20μM August 8
2295 / reverse primer for mutagenesis for 20μM August 8
2294 / forward primer for mutagenesis for 100μM August 8
2295 / reverse primer for mutagenesis for 100μM August 8

miscellaneous

part plasmid description status μl left concentration date
dNTP mixATP, GTP, TTP, CTP9410μMaug 10
pSB1A2medium constitutieve promoterjuly 23
ampicilline
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