Team:KULeuven/Freezer
From 2009.igem.org
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|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 pl.)||31 µl||76,0 ng/µl||August 12 | |{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 pl.)||31 µl||76,0 ng/µl||August 12 | ||
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|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech B)||42,8 µl||96,7 ng/µl||August 19 | |{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech B)||42,8 µl||96,7 ng/µl||August 19 | ||
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|{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech C)||48 µl||105,6 ng/µl||August 19 | |{{kulpart|BBa_I742113}}||pSB1A2||ech + rbs||purified plasmid (ech C)||48 µl||105,6 ng/µl||August 19 | ||
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|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs B)||43,6 µl||113,4 ng/µl||August 19 | |{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs B)||43,6 µl||113,4 ng/µl||August 19 | ||
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|{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs C)||48 µl||90,3 ng/µl||August 19 | |{{kulpart|BBa_I742115}}||pSB1A2||fcs + rbs||purified plasmid (fcs C)||48 µl||90,3 ng/µl||August 19 | ||
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|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 B)||44 µl||125,0 ng/µl||August 19 | |{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 B)||44 µl||125,0 ng/µl||August 19 | ||
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|{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 C)||48 µl||104,2 ng/µl||August 19 | |{{kulpart|BBa_K238007}}||pSB1A2||Sam5 + rbs||purified plasmid (sam5 C)||48 µl||104,2 ng/µl||August 19 | ||
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|{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 B)||40,1 µl||63,2 ng/µl||August 19 | |{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 B)||40,1 µl||63,2 ng/µl||August 19 |
Revision as of 09:07, 26 August 2009
Contents |
Blue light receptor
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
MC4100 | / | strain of E.coli | july 24 | |||
2171 | / | forward primer for | 90µl | 20μM | july 24 | |
2172 | / | reverse primer for | 90µl | 20μM | july 24 | |
2171 | / | forward primer for | 100μM | july 24 | ||
2172 | / | reverse primer for | 100μM | july 24 | ||
2260 | / | forward primer for | 45µl | 20μM | aug 10 | |
2261 | / | reverse primer for | 45µl | 20μM | aug 10 | |
2260 | / | forward primer for | 100μM | aug 10 | ||
2261 | / | reverse primer for | 100μM | aug 10 | ||
pSB1A2 | GFP + rbs + terminator | restricted with EcoRI and XbaI | july 29 | |||
pSB1A2 | GFP + LVA-tag + terminator | restricted with EcoRI and XbaI | july 29 | |||
/ | BLR promoter region (1:200 adn 1:500) | restricted with EcoRI | july 29 | |||
/ | PCR BLR promoter region (360bp) | 4 microcentrifuge tubes | july 30 | |||
pSB3K3 | kanamycine plasmide backbone | taken from plates | 13μl | aug 11 | ||
/ | Exact blue light promotor | pcr product | / | 123,3 ng/μl | aug 13 | |
/ | constitutive promotor | taken from plates | 13μl | aug 11 | ||
/ | Exact blue light promotor | cut with EcoRI and SpeI | 26μl | 27 ng/μl | aug 12 | |
/ | LigA: Exact blue light promotor ligated with E0240 in pSB1A2 | T4 DNA ligation | 18μl | aug 13 | ||
/ | Lig B: Exact blue light promotor ligated with K145015 in pSB1A2 | T4 DNA ligation | 18μl | aug 14 | ||
/ | Lig A: Exact blue light promotor ligated with E02040 in pSB1A2 (1,3,5,7,9) | cut with EcoRI and PstI | 5 x 18μl | aug 18 |
Key/antikey/lock
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
/ | GFP with LVA tag | purified | july 28 | |||
/ | tetR promoter | purified | july 28 |
vanillin synthesis
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
pSB1A2 | ech + rbs | purified plasmid (oranje) | 108,8 ng/µl | August 4 | ||
pSB1A2 | Sam5 + rbs, PstI site removed | purified plasmid (oranje) | 101,3 ng/µl | August 5 | ||
pSB1A2 | COMT | purified plasmid (oranje) | 122,9 ng/µl | August 5 | ||
pSB1A2 | fcs + rbs | purified plasmid (fcs pl.) | 29 µl | 57,2 ng/µl | August 12 | |
pSB1A2 | Sam5 + rbs | purified plasmid (sam5 pl.) | 31 µl | 76,0 ng/µl | August 12 | |
pSB1A2 | ech + rbs | purified plasmid (ech B) | 42,8 µl | 96,7 ng/µl | August 19 | |
pSB1A2 | ech + rbs | purified plasmid (ech C) | 48 µl | 105,6 ng/µl | August 19 | |
pSB1A2 | fcs + rbs | purified plasmid (fcs B) | 43,6 µl | 113,4 ng/µl | August 19 | |
pSB1A2 | fcs + rbs | purified plasmid (fcs C) | 48 µl | 90,3 ng/µl | August 19 | |
pSB1A2 | Sam5 + rbs | purified plasmid (sam5 B) | 44 µl | 125,0 ng/µl | August 19 | |
pSB1A2 | Sam5 + rbs | purified plasmid (sam5 C) | 48 µl | 104,2 ng/µl | August 19 | |
pSB1A2 | Sam8 + rbs | purified plasmid (sam8 B) | 40,1 µl | 63,2 ng/µl | August 19 | |
pSB1A2 | sam8 + sam5 | ligation (SAMS 21) | August 24 | |||
pSB1A2 | fcs + ech | ligation (EF 21) | August 24 |
vanillin receptor
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
2235 | / | forward primer for | 20μM | August 3 | ||
2238 | / | reverse primer for | 20μM | August 3 | ||
2235 | / | forward primer for | 100μM | August 3 | ||
2238 | / | reverse primer for | 100μM | August 3 | ||
2242 | / | forward primer for | 20μM | August 3 | ||
2243 | / | reverse primer for | 20μM | August 3 | ||
2242 | / | forward primer for | 100μM | August 3 | ||
2243 | / | reverse primer for | 100μM | August 3 | ||
2244 | / | forward primer for | 20μM | August 3 | ||
2245 | / | reverse primer for | 20μM | August 3 | ||
2244 | / | forward primer for | 100μM | August 3 | ||
2245 | / | reverse primer for | 100μM | August 3 | ||
2246 | / | forward primer for | 20μM | August 3 | ||
2247 | / | reverse primer for | 20μM | August 3 | ||
2246 | / | forward primer for | 100μM | August 3 | ||
2247 | / | reverse primer for | 100μM | August 3 | ||
2288 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
2289 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
2288 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
2289 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
2290 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
2291 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
2290 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
2291 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
2292 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
2293 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
2292 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
2293 | / | reverse primer for mutagenesis for | 100μM | August 8 | ||
2294 | / | forward primer for mutagenesis for | 20μM | August 8 | ||
2295 | / | reverse primer for mutagenesis for | 20μM | August 8 | ||
2294 | / | forward primer for mutagenesis for | 100μM | August 8 | ||
2295 | / | reverse primer for mutagenesis for | 100μM | August 8 |
miscellaneous
part | plasmid | description | status | μl left | concentration | date |
---|---|---|---|---|---|---|
dNTP mix | ATP, GTP, TTP, CTP | 94 | 10μM | aug 10 | ||
pSB1A2 | medium constitutieve promoter | july 23 | ||||
ampicilline |
Categories: Team:KULeuven/BioBricks | Team:KULeuven/BioBricks/BBa K238000 | Team:KULeuven/BioBricks/BBa K238013 | Team:KULeuven/BioBricks/BBa E0240 | Team:KULeuven/BioBricks/BBa K145015 | Team:KULeuven/BioBricks/BBa pSB3K3-P1010 | Team:KULeuven/BioBricks/BBa J23101 | Team:KULeuven/BioBricks/BBa K238013+ BBa E0240 | Team:KULeuven/BioBricks/BBa K238013+ BBa K145015 | Team:KULeuven/BioBricks/BBa K238013+ BBa E02040 | Team:KULeuven/BioBricks/BBa K145201 | Team:KULeuven/BioBricks/BBa I742113 | Team:KULeuven/BioBricks/BBa K238007 | Team:KULeuven/BioBricks/BBa I742107 | Team:KULeuven/BioBricks/BBa I742115 | Team:KULeuven/BioBricks/BBa I742106 | Team:KULeuven/BioBricks/BBa K238008 | Team:KULeuven/BioBricks/BBa K238009 | Team:KULeuven/BioBricks/BBa K238010 | Team:KULeuven/BioBricks/BBa K238011 | Team:KULeuven/BioBricks/J23110