Revision as of 09:29, 26 August 2009

Used Parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=KULeuven Submitted Parts]

Blue light receptor

part	plasmid	description	status	μl left	concentration	date
MC4100	/	strain of E.coli				july 24
2171	/	forward primer for		90µl	20μM	july 24
2172	/	reverse primer for		90µl	20μM	july 24
2171	/	forward primer for			100μM	july 24
2172	/	reverse primer for			100μM	july 24
2260	/	forward primer for		45µl	20μM	aug 10
2261	/	reverse primer for		45µl	20μM	aug 10
2260	/	forward primer for			100μM	aug 10
2261	/	reverse primer for			100μM	aug 10
	pSB1A2	GFP + rbs + terminator	restricted with EcoRI and XbaI			july 29
	pSB1A2	GFP + LVA-tag + terminator	restricted with EcoRI and XbaI			july 29
	/	BLR promoter region (1:200 adn 1:500)	restricted with EcoRI			july 29
	/	PCR BLR promoter region (360bp)	4 microcentrifuge tubes			july 30
	pSB3K3	kanamycine plasmide backbone	taken from plates	13μl		aug 11
	/	Exact blue light promotor	pcr product	/	123,3 ng/μl	aug 13
	/	constitutive promotor	taken from plates	13μl		aug 11
	/	Exact blue light promotor	cut with EcoRI and SpeI	26μl	27 ng/μl	aug 12
	/	LigA: Exact blue light promotor ligated with E0240 in pSB1A2	T4 DNA ligation	18μl		aug 13
	/	Lig B: Exact blue light promotor ligated with K145015 in pSB1A2	T4 DNA ligation	18μl		aug 14
	/	Lig A: Exact blue light promotor ligated with E02040 in pSB1A2 (1,3,5,7,9)	cut with EcoRI and PstI	5 x 18μl		aug 18

part	plasmid	description	status	μl left	concentration	date
	/	GFP with LVA tag	purified			july 28
	/	tetR promoter	purified			july 28

plasmid	description	status	μl left	concentration	date
pSB1A2	ech + rbs	purified plasmid (oranje)		108,8 ng/µl	August 4
pSB1A2	Sam5 + rbs, PstI site removed	purified plasmid (oranje)		101,3 ng/µl	August 5
pSB1A2	COMT	purified plasmid (oranje)		122,9 ng/µl	August 5
pSB1A2	fcs + rbs	purified plasmid (fcs pl.)	29 µl	57,2 ng/µl	August 12
pSB1A2	Sam5 + rbs	purified plasmid (sam5 pl.)	31 µl	76,0 ng/µl	August 12
pSB1A2	ech + rbs	purified plasmid (ech B)	42,8 µl	96,7 ng/µl	August 19
pSB1A2	ech + rbs	purified plasmid (ech C)	48 µl	105,6 ng/µl	August 19
pSB1A2	fcs + rbs	purified plasmid (fcs B)	43,6 µl	113,4 ng/µl	August 19
pSB1A2	fcs + rbs	purified plasmid (fcs C)	48 µl	90,3 ng/µl	August 19
pSB1A2	Sam5 + rbs	purified plasmid (sam5 B)	44 µl	125,0 ng/µl	August 19
pSB1A2	Sam5 + rbs	purified plasmid (sam5 C)	48 µl	104,2 ng/µl	August 19
pSB1A2	Sam8 + rbs	purified plasmid (sam8 B)	40,1 µl	63,2 ng/µl	August 19
pSB1A2	sam8 + sam5	ligation mix (SAMS)			August 24
pSB1A2	fcs + ech	ligation mix (EF)			August 24
pSB1A2	fcs + ech	purified ligation plasmid (EF I)	48 µl	53,9 ng/µl	August 4
pSB1A2	fcs + ech	purified ligation plasmid (EF II)	42 µl	39,4 ng/µl	August 4
pSB1A2	fcs + ech	purified ligation plasmid (EF III)	39,9 µl	57,1 ng/µl	August 4
pSB1A2	sam8 + sam5	purified ligation plasmid (SAMS I)	48 µl	76,8 ng/µl	August 4
pSB1A2	sam8 + sam5	purified ligation plasmid (SAMS II)	35,3	83,4 ng/µl	August 4
pSB1A2	sam8 + sam5	purified ligation plasmid (SAMS III)	39,3 µl	61,5 ng/µl	August 4

part	plasmid	description	concentration	date
2235	/	forward primer for	20μM	August 3
2238	/	reverse primer for	20μM	August 3
2235	/	forward primer for	100μM	August 3
2238	/	reverse primer for	100μM	August 3
2242	/	forward primer for	20μM	August 3
2243	/	reverse primer for	20μM	August 3
2242	/	forward primer for	100μM	August 3
2243	/	reverse primer for	100μM	August 3
2244	/	forward primer for	20μM	August 3
2245	/	reverse primer for	20μM	August 3
2244	/	forward primer for	100μM	August 3
2245	/	reverse primer for	100μM	August 3
2246	/	forward primer for	20μM	August 3
2247	/	reverse primer for	20μM	August 3
2246	/	forward primer for	100μM	August 3
2247	/	reverse primer for	100μM	August 3
2288	/	forward primer for mutagenesis for	20μM	August 8
2289	/	reverse primer for mutagenesis for	20μM	August 8
2288	/	forward primer for mutagenesis for	100μM	August 8
2289	/	reverse primer for mutagenesis for	100μM	August 8
2290	/	forward primer for mutagenesis for	20μM	August 8
2291	/	reverse primer for mutagenesis for	20μM	August 8
2290	/	forward primer for mutagenesis for	100μM	August 8
2291	/	reverse primer for mutagenesis for	100μM	August 8
2292	/	forward primer for mutagenesis for	20μM	August 8
2293	/	reverse primer for mutagenesis for	20μM	August 8
2292	/	forward primer for mutagenesis for	100μM	August 8
2293	/	reverse primer for mutagenesis for	100μM	August 8
2294	/	forward primer for mutagenesis for	20μM	August 8
2295	/	reverse primer for mutagenesis for	20μM	August 8
2294	/	forward primer for mutagenesis for	100μM	August 8
2295	/	reverse primer for mutagenesis for	100μM	August 8

part	plasmid	description	μl left	concentration	date
dNTP mix		ATP, GTP, TTP, CTP	94	10μM	aug 10
	pSB1A2	medium constitutieve promoter			july 23
ampicilline

@@ Line 106: / Line 106: @@
 |{{kulpart|BBa_I742106}}||pSB1A2||Sam8 + rbs||purified plasmid (sam8 B)||40,1 µl||63,2 ng/µl||August 19
 |-
-|||pSB1A2||sam8 + sam5||ligation mix (SAMS 21)||||||August 24
+|||pSB1A2||sam8 + sam5||ligation mix (SAMS)||||||August 24
 |-
-|||pSB1A2||fcs + ech||ligation mix (EF 21)||||||August 24
+|||pSB1A2||fcs + ech||ligation mix (EF)||||||August 24
 |-
 |||pSB1A2||fcs + ech||purified ligation plasmid (EF I)||48 µl||53,9 ng/µl||August 4