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Template:Team:KULeuven/25 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
| Vector || insert | | Vector || insert | ||
|- align="center" | |- align="center" | ||
| - | | {{kulpart| | + | | {{kulpart|psB1A3}}+ E0240 || {{kulpart|BBa_J23101}} || |
|- align="center" | |- align="center" | ||
| 30ng -> 1,4μl || 40,8ng -> 2μl | | 30ng -> 1,4μl || 40,8ng -> 2μl | ||
|- align="center" | |- align="center" | ||
|} | |} | ||
Revision as of 09:36, 1 September 2009
- miniprepping the liquid cultures with pSB3K3
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| pSB3K3 (1) | 27,6 | 1,91 | |
| pSB3K3 (2) | 47,7 | 2,08 |
- restriction digest of pSB3K3 with EcoRI and PstI
- gel electroforesis and extraction of the RD of pSB3K3
--> failed. there was no signal at all
- new colonies were ented in liquid culture.
- ligation C performed: J23101 (A) + E0240
| Vector | insert | |
| + E0240 | ||
| 30ng -> 1,4μl | 40,8ng -> 2μl |
