Template:Team:KULeuven/12 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
JochemDeen (Talk | contribs) |
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| Vector || insert | | Vector || insert | ||
|- align="center" | |- align="center" | ||
- | | {{kulpart|psB1A3}}+P1010 || {{kulpart| | + | | {{kulpart|psB1A3}}+P1010 || {{kulpart|BBa_K238013}} (BLP) |
|- align="center" | |- align="center" | ||
| 50ng -> 2,5 μl || 5 ng -> 0,3 μl | | 50ng -> 2,5 μl || 5 ng -> 0,3 μl | ||
|- align="center" | |- align="center" | ||
|} | |} |
Revision as of 09:37, 1 September 2009
- Gel electrophorese of BlP cut with EcoRI and SpeI
- Gel extraction of the Blp
Nanodrop results
Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
---|---|---|---|
Blp | 27 | 2,08 |
- PCR (with Pfx) of blue light promoter with primers iGEM 2260 and igem 2261
- Annealing of 58C
- Innoculate liquid medium
- Innoculation of Psb3k3-P1010 and J23101
- Ligation
Vector | insert |
+P1010 | (BLP) |
50ng -> 2,5 μl | 5 ng -> 0,3 μl |