Template:Team:KULeuven/12 August 2009/BlueLightReceptor

From 2009.igem.org

(Difference between revisions)
Line 17: Line 17:
| Vector || insert  
| Vector || insert  
|- align="center"
|- align="center"
-
| {{kulpart|psB1A3}}+P1010 || {{kulpart|bba_K238013}} (BLP)  
+
| {{kulpart|psB1A3}}+P1010 || {{kulpart|BBa_K238013}} (BLP)  
|- align="center"
|- align="center"
| 50ng -> 2,5 μl || 5 ng -> 0,3 μl  
| 50ng -> 2,5 μl || 5 ng -> 0,3 μl  
|- align="center"
|- align="center"
|}
|}

Revision as of 09:37, 1 September 2009

  1. Gel electrophorese of BlP cut with EcoRI and SpeI
  2. Gel extraction of the Blp

Nanodrop results

Part concentration (ng/μl) 260/280 λ 260/230 λ
Blp 27 2,08
  1. PCR (with Pfx) of blue light promoter with primers iGEM 2260 and igem 2261
    • Annealing of 58C
  2. Innoculate liquid medium
    • Innoculation of Psb3k3-P1010 and J23101
  3. Ligation
Vector insert
+P1010 (BLP)
50ng -> 2,5 μl 5 ng -> 0,3 μl
Retrieved from "http://2009.igem.org/Template:Team:KULeuven/12_August_2009/BlueLightReceptor"