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Template:Team:KULeuven/31 August 2009/VanillinProduction
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* Restriction digest of ech and fcs from saturday was tested and showed 6 signals with varying lengths for ech and 1 very large signal for fcs, both inconsistent. To test for errors in the restriction sites we cut each vector with 1 restriction digest for each of the sites. Giving a total of 4 tests for ech and 4 for fcs. | * Restriction digest of ech and fcs from saturday was tested and showed 6 signals with varying lengths for ech and 1 very large signal for fcs, both inconsistent. To test for errors in the restriction sites we cut each vector with 1 restriction digest for each of the sites. Giving a total of 4 tests for ech and 4 for fcs. | ||
** Restrction is in 37C overnight. | ** Restrction is in 37C overnight. | ||
| - | * For ligation of SAMS III RD (from thursday) and TER II we need 44,4 ul of SAMSIII which we dont have thus we cut two additional volumes with | + | * For ligation of SAMS III RD (from thursday) and TER II we need 44,4 ul of SAMSIII which we dont have thus we cut two additional volumes with SAMS I overnight |
** SAMS I A RD and SAMS I B RD | ** SAMS I A RD and SAMS I B RD | ||
Revision as of 11:04, 1 September 2009
- Electroporation of SAMS I, SAMS II AND EF II
- Restriction digest of ech and fcs from saturday was tested and showed 6 signals with varying lengths for ech and 1 very large signal for fcs, both inconsistent. To test for errors in the restriction sites we cut each vector with 1 restriction digest for each of the sites. Giving a total of 4 tests for ech and 4 for fcs.
- Restrction is in 37C overnight.
- For ligation of SAMS III RD (from thursday) and TER II we need 44,4 ul of SAMSIII which we dont have thus we cut two additional volumes with SAMS I overnight
- SAMS I A RD and SAMS I B RD
