Template:Team:KULeuven/1 September 2009/VanillinProduction

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(New page: * SAMS I B RD is used for ligation with TER II RD ** Since we want to avoid gel purification we use the direct sample from the restriction diget ** The restriction enzymes are denatured by...)
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  • SAMS I B RD is used for ligation with TER II RD
    • Since we want to avoid gel purification we use the direct sample from the restriction diget
    • The restriction enzymes are denatured by keeping the sample at 85C for 20 minutes
vector insert
Terminator SAMS
2000 bp 3070 bp
50 ng 280 ng
10 μl 16 μl
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