Template:Team:KULeuven/1 September 2009/VanillinProduction

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(Difference between revisions)
(New page: * SAMS I B RD is used for ligation with TER II RD ** Since we want to avoid gel purification we use the direct sample from the restriction diget ** The restriction enzymes are denatured by...)
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* The genes fcs and ech are individually cut with the four restriction enzymes
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{| border ="1" align="center"
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| EcoR1 || fcs RD-E
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|- align="center"
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| Spe1 || fcs RD-S
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|- align="center"
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| Pst1 || fcs RD-P
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|- align="center"
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| Xba1 || fcs RD-X
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|- align="center"
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| EcoR1 || Ech RD-E
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|- align="center"
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| Spe1 || Ech RD-S
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|- align="center"
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| Pst1 || Ech RD-P
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|- align="center"
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| Xba1 || Ech RD-X
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|-
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|}
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* Results on gel shows that all the genes have the same length and show 1 line.
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* SAMS I A RD is put on gel, to be used for gel extraction and a part of SAMS I B RD is put on gel to check the length.
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** No signal... grrr...

Revision as of 11:11, 1 September 2009

  • SAMS I B RD is used for ligation with TER II RD
    • Since we want to avoid gel purification we use the direct sample from the restriction diget
    • The restriction enzymes are denatured by keeping the sample at 85C for 20 minutes
vector insert
Terminator SAMS
2000 bp 3070 bp
50 ng 280 ng
10 μl 16 μl
  • The genes fcs and ech are individually cut with the four restriction enzymes
EcoR1 fcs RD-E
Spe1 fcs RD-S
Pst1 fcs RD-P
Xba1 fcs RD-X
EcoR1 Ech RD-E
Spe1 Ech RD-S
Pst1 Ech RD-P
Xba1 Ech RD-X
  • Results on gel shows that all the genes have the same length and show 1 line.
  • SAMS I A RD is put on gel, to be used for gel extraction and a part of SAMS I B RD is put on gel to check the length.
    • No signal... grrr...
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