Template:Team:KULeuven/25 August 2009/BlueLightReceptor

From 2009.igem.org

(Difference between revisions)
Line 1: Line 1:
-
* miniprepping the liquid cultures with pSB3K3
+
* miniprepping the liquid cultures with {{kulpart|pSB3K3}}
{| border ="1" align="center"
{| border ="1" align="center"
!| Part || concentration (ng/μl) || 260/280 λ ||  
!| Part || concentration (ng/μl) || 260/280 λ ||  
|- align="center"
|- align="center"
-
| pSB3K3 (1) || 27,6 || 1,91 ||  
+
| {{kulpart|pSB3K3}} (1) || 27,6 || 1,91 ||  
|- align="center"
|- align="center"
-
| pSB3K3 (2) || 47,7 || 2,08 ||   
+
| {{kulpart|pSB3K3}} (2) || 47,7 || 2,08 ||   
|}
|}
-
*restriction digest of pSB3K3 with EcoRI and PstI
+
*restriction digest of {{kulpart|pSB3K3}} with EcoRI and PstI
-
*gel electroforesis and extraction of the RD of pSB3K3
+
*gel electroforesis and extraction of the RD of {{kulpart|pSB3K3}}
--> failed. there was no signal at all
--> failed. there was no signal at all
*new colonies were ented in liquid culture.
*new colonies were ented in liquid culture.
-
*ligation C performed: J23101 (A) + E0240
+
*ligation C performed: {{kulpart|BBa_J23101}} (A) + {{kulpart|BBa_E0240}}
{| border ="1" align="center"
{| border ="1" align="center"
|- align="center"
|- align="center"
| Vector || insert  
| Vector || insert  
|- align="center"
|- align="center"
-
| {{kulpart|psB1A3}}+ E0240 || {{kulpart|BBa_J23101}} ||  
+
| {{kulpart|psB1A3}} + {{kulpart|BBa_E0240}} || {{kulpart|BBa_J23101}} ||  
|- align="center"
|- align="center"
| 30ng -> 1,4μl || 40,8ng -> 2μl  
| 30ng -> 1,4μl || 40,8ng -> 2μl  
|- align="center"
|- align="center"
|}
|}

Revision as of 11:55, 1 September 2009

  • miniprepping the liquid cultures with
Part concentration (ng/μl) 260/280 λ
(1) 27,6 1,91
(2) 47,7 2,08
  • restriction digest of with EcoRI and PstI
  • gel electroforesis and extraction of the RD of

--> failed. there was no signal at all

  • new colonies were ented in liquid culture.
  • ligation C performed: (A) +
Vector insert
+
30ng -> 1,4μl 40,8ng -> 2μl
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