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Template:Team:KULeuven/26 August 2009/BlueLightReceptor
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| - | 4. two electroporations were performed and plated on LB medium. one with ligC ({{kulpart|BBa_J23101}} + {{kulpart|BBa_E0240}}) and one with pSB3K3 DNA. | + | 4. two electroporations were performed and plated on LB medium. one with ligC ({{kulpart|BBa_J23101}} + {{kulpart|BBa_E0240}}) and one with {{kulpart|pSB3K3}} DNA. |
Revision as of 11:56, 1 September 2009
- the plates with ligation A (blp + GFP) where fetched from the blue light installation. there was no GFP signal. the following actions will be taken:
- the plasmids will be purified from the colonies and will be sequenced using primer 2260.
- next time we will probably put them in liquid cultures under the LEDs while shaking gently. also, other parameters that need to be considered are being researched.
- they will not be exposed to the light as long anymore. we decided that 1h will be more than enough.
- possible bleaching?
- a colony from all three plates with the ligA-construct were taken and ented in liquid culture. this was needed to check wether the colonies were still alive.
- culture 13/08
- culture 14/08 (1)
- culture 14/08 (2)
- by 5pm, one of the liquid cultures had grown and was miniprepped. 20μl was sent for sequencing together with 5μM of primer 2260.
| Part | concentration (ng/μl) | 260/280 λ | |
|---|---|---|---|
| ligA (14/08 (1)) | 23,1 | 2,21 |
4. two electroporations were performed and plated on LB medium. one with ligC ( + ) and one with DNA.
