"
Template:Team:KULeuven/13 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 11: | Line 11: | ||
|} | |} | ||
# Restriction digest {{kulpart|BBa_K145015}} cut with EcoRI and XbaI | # Restriction digest {{kulpart|BBa_K145015}} cut with EcoRI and XbaI | ||
| - | # Electroporation of {{kulpart| | + | # Electroporation of {{kulpart|pSB3K3}} + {{kulpart|BBa_P1010}} and the ligation of ... |
# Gelelectrophorese of {{Kulpart|BBa_K145015}} | # Gelelectrophorese of {{Kulpart|BBa_K145015}} | ||
#* Only 1 band, so probably everything was cut | #* Only 1 band, so probably everything was cut | ||
Revision as of 12:04, 1 September 2009
- PCR product of 12-august of pairt
- Miniprep of
Nanodrop results:
| Part | concentration (ng/μl) | 260/280 λ | 260/230 λ |
|---|---|---|---|
| 123,3 | 1,82 | ||
| 141,2 | 1,99 |
- Restriction digest cut with EcoRI and XbaI
- Electroporation of + and the ligation of ...
- Gelelectrophorese of
- Only 1 band, so probably everything was cut
- Gelectraction of
- Nanodrop = 29,3 ng/μl
- ligation of vector and insert
