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Template:Team:KULeuven/25 August 2009/BlueLightReceptor
From 2009.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
* miniprepping the liquid cultures with {{kulpart|pSB3K3}} | * miniprepping the liquid cultures with {{kulpart|pSB3K3}} | ||
{| border ="1" align="center" | {| border ="1" align="center" | ||
| - | !| Part || concentration (ng/μl) || 260/280 λ | + | !| Part || concentration (ng/μl) || 260/280 λ |
|- align="center" | |- align="center" | ||
| - | | {{kulpart|pSB3K3}} (1) || 27,6 || 1,91 | + | | {{kulpart|pSB3K3}} (1) || 27,6 || 1,91 |
|- align="center" | |- align="center" | ||
| - | | {{kulpart|pSB3K3}} (2) || 47,7 || 2,08 | + | | {{kulpart|pSB3K3}} (2) || 47,7 || 2,08 |
|} | |} | ||
*restriction digest of {{kulpart|pSB3K3}} with EcoRI and PstI | *restriction digest of {{kulpart|pSB3K3}} with EcoRI and PstI | ||
| Line 16: | Line 16: | ||
| Vector || insert | | Vector || insert | ||
|- align="center" | |- align="center" | ||
| - | | {{kulpart|pSB1A3}} + {{kulpart|BBa_E0240}} || {{kulpart|BBa_J23101}} | + | | {{kulpart|pSB1A3}} + {{kulpart|BBa_E0240}} || {{kulpart|BBa_J23101}} |
|- align="center" | |- align="center" | ||
| 30ng -> 1,4μl || 40,8ng -> 2μl | | 30ng -> 1,4μl || 40,8ng -> 2μl | ||
|- align="center" | |- align="center" | ||
|} | |} | ||
Revision as of 12:13, 1 September 2009
- miniprepping the liquid cultures with
| Part | concentration (ng/μl) | 260/280 λ |
|---|---|---|
| (1) | 27,6 | 1,91 |
| (2) | 47,7 | 2,08 |
- restriction digest of with EcoRI and PstI
- gel electroforesis and extraction of the RD of
--> failed. there was no signal at all
- new colonies were ented in liquid culture.
- ligation C performed: (A) +
| Vector | insert |
| + | |
| 30ng -> 1,4μl | 40,8ng -> 2μl |
