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Template:Team:KULeuven/30 July 2009/VanillinProduction
From 2009.igem.org
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Following parts were put in competent cells by electroporation: | Following parts were put in competent cells by electroporation: | ||
| - | *RBS + ech, in pSB1A2, {{kulpart|BBa_I742113}} | + | *RBS + ech, in {{kulpart|pSB1A2}}, {{kulpart|BBa_I742113}} |
| - | *RBS + fcs, in pSB1A2, {{kulpart|BBa_I742115}} | + | *RBS + fcs, in {{kulpart|pSB1A2}}, {{kulpart|BBa_I742115}} |
The cells were then put in liquid LB medium to recuperate from the procedure. After a few hours they were transfered on LB/amp plates and grown overnight at 37°C. | The cells were then put in liquid LB medium to recuperate from the procedure. After a few hours they were transfered on LB/amp plates and grown overnight at 37°C. | ||
Revision as of 12:34, 1 September 2009
Following parts were put in competent cells by electroporation:
- RBS + ech, in ,
- RBS + fcs, in ,
The cells were then put in liquid LB medium to recuperate from the procedure. After a few hours they were transfered on LB/amp plates and grown overnight at 37°C.
