Team:Newcastle/Project/Labwork/OurProtocols/PCR Products
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(New page: *Use PCR Clean-up kit to clear the PCR product This should result in 50ul of final product *Measure the concentration of the product This step will will be useful to determine how much of ...)
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(New page: *Use PCR Clean-up kit to clear the PCR product This should result in 50ul of final product *Measure the concentration of the product This step will will be useful to determine how much of ...)
Newer edit →
Revision as of 14:23, 1 September 2009
- Use PCR Clean-up kit to clear the PCR product
This should result in 50ul of final product
- Measure the concentration of the product
This step will will be useful to determine how much of the product to use for the ligation
- Digest the PCR product
Although the total will be 50ul, some of it will be consumed for the gel and to measure the concentration