Team:Newcastle/Project/Labwork/OurProtocols/PCR Products

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(New page: *Use PCR Clean-up kit to clear the PCR product This should result in 50ul of final product *Measure the concentration of the product This step will will be useful to determine how much of ...)
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Revision as of 14:23, 1 September 2009

  • Use PCR Clean-up kit to clear the PCR product

This should result in 50ul of final product

  • Measure the concentration of the product

This step will will be useful to determine how much of the product to use for the ligation

  • Digest the PCR product

Although the total will be 50ul, some of it will be consumed for the gel and to measure the concentration