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Team:Wash U/Calendar-Home/1 September 2009
From 2009.igem.org
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| - | Did 2 digests of RBS30_cph8 with 1.5 ug each according to prefix-insertion instructions from the Silver lab- cut with EcoRI and PstI | + | Did 2 digests of RBS30_cph8 with 1.5 ug each according to prefix-insertion instructions from the Silver lab- cut with EcoRI and PstI (A1 and A2) |
| - | Did 1 digest each of 1.5 ug of ompR_term_ompC_GFP and ompR_term_ompC_pucB/A as the vector for prefix insertion- cut with EcoRI and XbaI. | + | Did 1 digest each of 1.5 ug of ompR_term_ompC_GFP (B) and ompR_term_ompC_pucB/A (C) as the vector for prefix insertion- cut with EcoRI and XbaI. |
The Results of this digest are: | The Results of this digest are: | ||
| + | |||
| + | The first 4 lanes are the two rxns for RBS30_cph8. The top band is the one that was cutout (ab. 2.3 kb) | ||
| + | |||
| + | The next 2 lanes are ompR_term_ompC_GFP and the last two are ompR_term_ompC_pucB/A | ||
[[Image:9_1 gel purify.jpg]] | [[Image:9_1 gel purify.jpg]] | ||
| - | |||
| - | The | + | |
| + | Concentrations: of products after Gel purification: | ||
| + | |||
| + | A1) A2) B) C) | ||
| + | |||
| + | |||
| + | The products were ligated in the following ratios after gel purification for 1 hr at 22 deg C, followed by 20 min at 80 deg C: | ||
Revision as of 21:06, 1 September 2009
9/1 Minipreps
| number | notation | concentration |
| A | RBS30_cph8 | 168.0 |
| B | ompR_term_ompC_GFP | 161.0 |
| C | ompR_term_ompC_pucB/A | 188.0 |
Did 2 digests of RBS30_cph8 with 1.5 ug each according to prefix-insertion instructions from the Silver lab- cut with EcoRI and PstI (A1 and A2)
Did 1 digest each of 1.5 ug of ompR_term_ompC_GFP (B) and ompR_term_ompC_pucB/A (C) as the vector for prefix insertion- cut with EcoRI and XbaI.
The Results of this digest are:
The first 4 lanes are the two rxns for RBS30_cph8. The top band is the one that was cutout (ab. 2.3 kb)
The next 2 lanes are ompR_term_ompC_GFP and the last two are ompR_term_ompC_pucB/A
Concentrations: of products after Gel purification:
A1) A2) B) C)
The products were ligated in the following ratios after gel purification for 1 hr at 22 deg C, followed by 20 min at 80 deg C:
