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Team:UNC Chapel Hill/20 May 2009
From 2009.igem.org
(Difference between revisions)
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[[Team:UNC_Chapel_Hill/Notebook|Back]] | [[Team:UNC_Chapel_Hill/Notebook|Back]] | ||
| - | + | # Shawn went to the iGem advisor workshop this past weekend. | |
| - | + | # The site 2009.igem.org has been updated and is being updated constantly. | |
| - | constantly. | + | # We have received the DNA Distribution kit in the mail. 1000 of |
| - | + | ||
the post popular parts were given. A list of the parts can be seen | the post popular parts were given. A list of the parts can be seen | ||
here: http://partsregistry.org/Main_Page | here: http://partsregistry.org/Main_Page | ||
| - | + | # As part of the project, we will need to keep a lab notebook. | |
This is part of the iGem site in the Team wiki: https://igem.org/Team_Wikis?year=2009 | This is part of the iGem site in the Team wiki: https://igem.org/Team_Wikis?year=2009 | ||
(Be sure to make an account if you haven't done so already) | (Be sure to make an account if you haven't done so already) | ||
| - | + | # Slides and the video from the workshop will be posted soon. | |
These will be important. | These will be important. | ||
| - | + | # Shawn was able to get a feel for the other teams in terms of | |
their resources. Most of the teams were like us; didn't really have | their resources. Most of the teams were like us; didn't really have | ||
funding and trying to get by. Other teams are better with a | funding and trying to get by. Other teams are better with a | ||
consistent source of funding. And others are worse; going to try to | consistent source of funding. And others are worse; going to try to | ||
do everything when they come back in the fall. | do everything when they come back in the fall. | ||
| - | + | # You can do this project and get APPL 395 credit. | |
| - | + | # Our main goal right now is to get a project idea developed and | |
finalized. We want to start simple for this year. | finalized. We want to start simple for this year. | ||
| - | + | # Here are the results from last year: https://igem.org/Results?year=2008 | |
. The most sophisticated project was probably Team Slovenia: | . The most sophisticated project was probably Team Slovenia: | ||
https://2008.igem.org/Team:Slovenia We probably do not want to do | https://2008.igem.org/Team:Slovenia We probably do not want to do | ||
something like this (way too complicated). The ideal project will be | something like this (way too complicated). The ideal project will be | ||
very student driven. | very student driven. | ||
| - | + | # We can make our own new part. This would then be added to the | |
iGem registry. | iGem registry. | ||
| - | + | # Prizes are awarded based mainly on well the project is | |
characterized. Sophistication is not too important. | characterized. Sophistication is not too important. | ||
| - | + | # We will be doing some form of transformation, most likely with | |
E. Coli. | E. Coli. | ||
| - | + | # In order to help narrow in on a project, be sure to check out | |
the previous year results and look at presentations and posters: | the previous year results and look at presentations and posters: | ||
https://igem.org/Results?year=2008 | https://igem.org/Results?year=2008 | ||
| - | + | # Scott suggested the book, Molecular cloning By Joseph Sambrook, | |
for background information. Available in Scott's lab. We'll be doing | for background information. Available in Scott's lab. We'll be doing | ||
informal reviews and lectures throughout the summer to get everyone up | informal reviews and lectures throughout the summer to get everyone up | ||
to speed in terms of learning the material. | to speed in terms of learning the material. | ||
| - | + | # The most simple project that we can do, just to see if we can | |
get it working, is doing a RFP transformation in E. Coli. This would | get it working, is doing a RFP transformation in E. Coli. This would | ||
entail getting the gene for RFP and a promoter to see if we can the | entail getting the gene for RFP and a promoter to see if we can the | ||
E.Coli to fluoresce red. | E.Coli to fluoresce red. | ||
| - | + | # Parts are clear, defined components (think like circuit | |
components). They follow particular standards. Understanding parts | components). They follow particular standards. Understanding parts | ||
will be very important to the competition. | will be very important to the competition. | ||
| - | + | # For project ideas, don't worry too much about another team doing | |
the same thing. | the same thing. | ||
| - | + | # Examples: BBa_R0011 - this is a basic promoter for E. Coli. | |
Here is the specific page: http://partsregistry.org/Part:BBa_R0011 | Here is the specific page: http://partsregistry.org/Part:BBa_R0011 | ||
If you click through the page, you'll notice things such as the DNA | If you click through the page, you'll notice things such as the DNA | ||
| Line 53: | Line 52: | ||
for, standards needed, and etc. Stars indicate parts that we have | for, standards needed, and etc. Stars indicate parts that we have | ||
already. | already. | ||
| - | + | # Gingko Bioworks (http://ginkgobioworks.com/) is a company that | |
started based on the iGem Synthetic biology premise: standardized | started based on the iGem Synthetic biology premise: standardized | ||
parts for biology | parts for biology | ||
| - | + | # Need to understand that there is a public perception that may be | |
different from what a researcher may see. Important to be cognizant | different from what a researcher may see. Important to be cognizant | ||
about. | about. | ||
| - | + | # Safety. May need to follow some protocol. | |
| - | + | # Should schedule a regular, (at least) weekly meeting. | |
| - | + | # Project is basically due 2 weeks before the Jamboree | |
| - | + | # Funding. | |
| - | + | # Need to set up an account to get funding. | |
| - | + | # It's okay if you don't understand everything right now. Feel | |
free to interrupt or inform anyone else if you need clarification. | free to interrupt or inform anyone else if you need clarification. | ||
Revision as of 01:33, 5 June 2009
- Shawn went to the iGem advisor workshop this past weekend.
- The site 2009.igem.org has been updated and is being updated constantly.
- We have received the DNA Distribution kit in the mail. 1000 of
the post popular parts were given. A list of the parts can be seen here: http://partsregistry.org/Main_Page
- As part of the project, we will need to keep a lab notebook.
This is part of the iGem site in the Team wiki: https://igem.org/Team_Wikis?year=2009
(Be sure to make an account if you haven't done so already)
- Slides and the video from the workshop will be posted soon.
These will be important.
- Shawn was able to get a feel for the other teams in terms of
their resources. Most of the teams were like us; didn't really have funding and trying to get by. Other teams are better with a consistent source of funding. And others are worse; going to try to do everything when they come back in the fall.
- You can do this project and get APPL 395 credit.
- Our main goal right now is to get a project idea developed and
finalized. We want to start simple for this year.
- Here are the results from last year: https://igem.org/Results?year=2008
. The most sophisticated project was probably Team Slovenia: https://2008.igem.org/Team:Slovenia We probably do not want to do something like this (way too complicated). The ideal project will be very student driven.
- We can make our own new part. This would then be added to the
iGem registry.
- Prizes are awarded based mainly on well the project is
characterized. Sophistication is not too important.
- We will be doing some form of transformation, most likely with
E. Coli.
- In order to help narrow in on a project, be sure to check out
the previous year results and look at presentations and posters: https://igem.org/Results?year=2008
- Scott suggested the book, Molecular cloning By Joseph Sambrook,
for background information. Available in Scott's lab. We'll be doing informal reviews and lectures throughout the summer to get everyone up to speed in terms of learning the material.
- The most simple project that we can do, just to see if we can
get it working, is doing a RFP transformation in E. Coli. This would entail getting the gene for RFP and a promoter to see if we can the E.Coli to fluoresce red.
- Parts are clear, defined components (think like circuit
components). They follow particular standards. Understanding parts will be very important to the competition.
- For project ideas, don't worry too much about another team doing
the same thing.
- Examples: BBa_R0011 - this is a basic promoter for E. Coli.
Here is the specific page: http://partsregistry.org/Part:BBa_R0011 If you click through the page, you'll notice things such as the DNA sequence, the parameters need for the device, chassis that it works for, standards needed, and etc. Stars indicate parts that we have already.
- Gingko Bioworks (http://ginkgobioworks.com/) is a company that
started based on the iGem Synthetic biology premise: standardized parts for biology
- Need to understand that there is a public perception that may be
different from what a researcher may see. Important to be cognizant about.
- Safety. May need to follow some protocol.
- Should schedule a regular, (at least) weekly meeting.
- Project is basically due 2 weeks before the Jamboree
- Funding.
- Need to set up an account to get funding.
- It's okay if you don't understand everything right now. Feel
free to interrupt or inform anyone else if you need clarification.
