Revision as of 14:08, 3 September 2009

Used Parts
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=KULeuven Submitted Parts]

Blue light receptor

part	plasmid	description	status	μl left	concentration	date
MC4100	/	strain of E.coli				july 24
2260	/	forward primer for		42,5µl	20μM	aug 10
2261	/	reverse primer for		45µl	20μM	aug 10
		GFP + rbs + terminator	Miniprepped	100μl	85,6ng/μl	july 29
		GFP + rbs + terminator	restricted with EcoRI and XbaI	20μl		july 29
		GFP + LVA-tag + terminator	restricted with EcoRI and XbaI	30μl	29,3 ng/μl	aug 14
		kanamycine plasmide backbone	taken from plates	13μl		aug 11
	/	Exact blue light promotor	pcr product	20μl	123,3 ng/μl	aug 13
	/	constitutive promotor	taken from plates	13μl		aug 11
	/	Exact blue light promotor	cut with EcoRI and SpeI	26μl	27 ng/μl	aug 12
+		LigA: Exact blue light promotor ligated with in	T4 DNA ligation	18μl		aug 13
+		Lig B: Exact blue light promotor ligated with in	T4 DNA ligation	18μl		aug 14
+	/	Lig A: Exact blue light promotor ligated with in (1,3,7,9)	cut with EcoRI and PstI	4 x 18μl		aug 24
+		LigA: Exact blue light promotor ligated with in	Miniprep (4x)	20μl		aug 18
		constitutive promotor	miniprep	10μl	141,2ng/μl	aug 14
(A)		constitutive promotor	restriction digest with EcoRI en SpeI	20μl	20,8ng/μl	aug 24
(B)		constitutive promotor	restriction digest with EcoRI en SpeI	10μl	12,8ng/μl	aug 24
+		LigA (1): Exact blue light promotor ligated with in	Miniprep	45μl	110,6ng/μl	aug 31
+		LigA (2): Exact blue light promotor ligated with in	Miniprep	43μl	76,7ng/μl	aug 31
+		LigA (3): Exact blue light promotor ligated with in	Miniprep	44μl	84,8ng/μl	aug 31
+		LigA (4): Exact blue light promotor ligated with in	Miniprep	34μl	31,7ng/μl	aug 31
	pSB1A2	RBS + GFP + terminator	Miniprep	45μl	104,3ng/μl	aug 31
(A)	pSB1A2	promotor	Miniprep	48μl	238,9ng/μl	sept 2
(B)	pSB1A2	promotor	Miniprep	48μl	248,8ng/μl	sept 2

Key/antikey/lock

part	plasmid	description	status	μl left	concentration	date
	/	GFP with LVA tag	purified			july 28
	/	tetR promoter	purified			july 28

vanillin synthesis

description	status	μl left	concentration	date
ech + rbs	purified plasmid (oranje)		108,8 ng/µl	August 4
Sam5 + rbs, PstI site removed	purified plasmid (oranje)		101,3 ng/µl	August 5
COMT	purified plasmid (oranje)		122,9 ng/µl	August 5
fcs + rbs	purified plasmid (fcs pl.)	29 µl	57,2 ng/µl	August 12
Sam5 + rbs	purified plasmid (sam5 pl.)	31 µl	76,0 ng/µl	August 12
ech + rbs	purified plasmid (ech B)	42,8 µl	96,7 ng/µl	August 19
ech + rbs	purified plasmid (ech C)	48 µl	105,6 ng/µl	August 19
fcs + rbs	purified plasmid (fcs B)	43,6 µl	113,4 ng/µl	August 19
fcs + rbs	purified plasmid (fcs C)	48 µl	90,3 ng/µl	August 19
Sam5 + rbs	purified plasmid (sam5 B)	44 µl	125,0 ng/µl	August 19
Sam5 + rbs	purified plasmid (sam5 C)	48 µl	104,2 ng/µl	August 19
Sam8 + rbs	purified plasmid (sam8 B)	40,1 µl	63,2 ng/µl	August 19
sam8 + sam5	ligation mix (SAMS)			August 24
fcs + ech	ligation mix (EF)			August 24
fcs + ech	purified ligation plasmid (EF I)	48 µl	53,9 ng/µl	August 4
fcs + ech	purified ligation plasmid (EF II)	42 µl	39,4 ng/µl	August 4
fcs + ech	purified ligation plasmid (EF III)	39,9 µl	57,1 ng/µl	August 4
sam8 + sam5	purified ligation plasmid (SAMS I)	48 µl	76,8 ng/µl	August 4
sam8 + sam5	purified ligation plasmid (SAMS II)	35,3	83,4 ng/µl	August 4
sam8 + sam5	purified ligation plasmid (SAMS III)	39,3 µl	61,5 ng/µl	August 4
sam8 + sam5	purified ligation plasmid (SAMS II), not purified from gel	5 µl		August 31
sam8 + sam5	purified ligation plasmid (SAMS I), purified from gel	25 µl		August 31
fcs + ech	purified ligation plasmid (EF II), purified from gel	5 µl		August 31
sam8 + sam5	SAMS 1 (from 'mother plate')	16 µl	35,4 ng/µl	September 3
sam8 + sam5	SAMS 2 (from 'mother plate')	19,9 µl	49,3 ng/µl	September 3
sam8 + sam5	SAMS 3 (from 'mother plate')	31,9 µl	62,0 ng/µl	September 3
sam8 + sam5	SAMS 4 (from 'mother plate')	33,6 µl	78,3 ng/µl	September 3
Terminator	Terminator, B0015	±20 µl	181,4	September 3

vanillin receptor

part	plasmid	description	concentration	date
2235	/	forward primer for	20μM	August 3
2238	/	reverse primer for	20μM	August 3
2235	/	forward primer for	100μM	August 3
2238	/	reverse primer for	100μM	August 3
2242	/	forward primer for	20μM	August 3
2243	/	reverse primer for	20μM	August 3
2242	/	forward primer for	100μM	August 3
2243	/	reverse primer for	100μM	August 3
2244	/	forward primer for	20μM	August 3
2245	/	reverse primer for	20μM	August 3
2244	/	forward primer for	100μM	August 3
2245	/	reverse primer for	100μM	August 3
2246	/	forward primer for	20μM	August 3
2247	/	reverse primer for	20μM	August 3
2246	/	forward primer for	100μM	August 3
2247	/	reverse primer for	100μM	August 3
2288	/	forward primer for mutagenesis for	20μM	August 8
2289	/	reverse primer for mutagenesis for	20μM	August 8
2288	/	forward primer for mutagenesis for	100μM	August 8
2289	/	reverse primer for mutagenesis for	100μM	August 8
2290	/	forward primer for mutagenesis for	20μM	August 8
2291	/	reverse primer for mutagenesis for	20μM	August 8
2290	/	forward primer for mutagenesis for	100μM	August 8
2291	/	reverse primer for mutagenesis for	100μM	August 8
2292	/	forward primer for mutagenesis for	20μM	August 8
2293	/	reverse primer for mutagenesis for	20μM	August 8
2292	/	forward primer for mutagenesis for	100μM	August 8
2293	/	reverse primer for mutagenesis for	100μM	August 8
2294	/	forward primer for mutagenesis for	20μM	August 8
2295	/	reverse primer for mutagenesis for	20μM	August 8
2294	/	forward primer for mutagenesis for	100μM	August 8
2295	/	reverse primer for mutagenesis for	100μM	August 8

miscellaneous

part	description	μl left	concentration	date
dNTP mix	ATP, GTP, TTP, CTP	94	10μM	aug 10
	medium constitutieve promoter			july 23
ampicilline

@@ Line 136: / Line 136: @@
 |||{{kulpart|pSB1A2}}||fcs + ech||purified ligation plasmid (EF II), purified from gel||5 µl|| ||August 31
 |-
-|||{{kulpart|pSB1A2}}||sam8 + sam5||SAMS 1 (from 'mother plate')||35,3||35,4 ng/µl||September 3
+|||{{kulpart|pSB1A2}}||sam8 + sam5||SAMS 1 (from 'mother plate')||16 µl ||35,4 ng/µl||September 3
 |-
-|||{{kulpart|pSB1A2}}||sam8 + sam5||SAMS 2 (from 'mother plate')||39,3 µl||49,3 ng/µl|| September 3
+|||{{kulpart|pSB1A2}}||sam8 + sam5||SAMS 2 (from 'mother plate')||19,9 µl||49,3 ng/µl|| September 3
 |-
-|||{{kulpart|pSB1A2}}||sam8 + sam5||SAMS 3 (from 'mother plate')||5 µl||62,0 ng/µl|| September 3
+|||{{kulpart|pSB1A2}}||sam8 + sam5||SAMS 3 (from 'mother plate')||31,9 µl||62,0 ng/µl|| September 3
 |-
-|||{{kulpart|pSB1A2}}||sam8 + sam5||SAMS 4 (from 'mother plate')||25 µl||78,3 ng/µl|| September 3
+|||{{kulpart|pSB1A2}}||sam8 + sam5||SAMS 4 (from 'mother plate')||33,6 µl||78,3 ng/µl|| September 3
 |-
 |{{kulpart|BBa__B0015}}||{{kulpart|pSB1A2}}||Terminator||Terminator, B0015 || ±20 µl||181,4|| September 3

Team:KULeuven/Freezer

From 2009.igem.org

Revision as of 14:08, 3 September 2009

Contents

Blue light receptor

Key/antikey/lock

vanillin synthesis

vanillin receptor

miscellaneous