Template:Team:KULeuven/3 September 2009/BlueLightReceptor

From 2009.igem.org

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# a restriction digest on {{kulpart|BBa_J23101}} with EcoRI and SpeI was performed. this will cut the promotor out of its vector ({{kulpart|BBa_J61002}}) which has a RFP reporter gene. we will put {{kulpart|BBa_K238013}} (cut with EcoRI and SpeI) in this vector. This way we will get a construct where RFP will be produced dependend on our blue light promotor. we can then compare this with the intensity of RFP produced by {{kulpart|BBa_J23101}}.
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# A restriction digest on {{kulpart|BBa_J23101}} with EcoRI and SpeI was performed. This will cut the promotor out of its vector ({{kulpart|BBa_J61002}}) which has a RFP reporter gene. We will put {{kulpart|BBa_K238013}} (cut with EcoRI and SpeI) in this vector. This way we will get a construct where RFP will be produced dependant on our blue light promotor. We can then compare this with the intensity of RFP produced by {{kulpart|BBa_J23101}}.
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# glycerol stock was made from LigA and {{kulpart|BBa_J23101}}. they are stored in the -80°C in S&P F8 67-68 and 69-70.
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# Glycerol stock was made from LigA and {{kulpart|BBa_J23101}}. They are stored in the -80°C in S&P F8 67-68 and 69-70.
# Ecoli strain DB3.1 cells were made competent for electroporesis.  
# Ecoli strain DB3.1 cells were made competent for electroporesis.  
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# gel electorforesis and extraction on {{kulpart|BBa_J23101}}
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# Gel electorphoresis and extraction on {{kulpart|BBa_J23101}}

Latest revision as of 06:38, 4 September 2009

  1. A restriction digest on with EcoRI and SpeI was performed. This will cut the promotor out of its vector () which has a RFP reporter gene. We will put (cut with EcoRI and SpeI) in this vector. This way we will get a construct where RFP will be produced dependant on our blue light promotor. We can then compare this with the intensity of RFP produced by .
  2. Glycerol stock was made from LigA and . They are stored in the -80°C in S&P F8 67-68 and 69-70.
  3. Ecoli strain DB3.1 cells were made competent for electroporesis.
  4. Gel electorphoresis and extraction on
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