"
Team:KULeuven/10 August 2009
From 2009.igem.org
(Difference between revisions)
JochemDeen (Talk | contribs) (New page: {{Team:KULeuven/Common/BeginHeader}} {{Team:KULeuven/Common/SubMenu_Project}} {{Team:KULeuven/Common/EndHeader}} {{Team:KULeuven/Notebook/DayNavigator}} = Project progress = <!-- Don't ...) |
Bart Bosmans (Talk | contribs) |
||
| Line 1: | Line 1: | ||
{{Team:KULeuven/Common/BeginHeader}} | {{Team:KULeuven/Common/BeginHeader}} | ||
| - | {{Team:KULeuven/Common/ | + | {{Team:KULeuven/Common/SubMenu_Notebook}} |
{{Team:KULeuven/Common/EndHeader}} | {{Team:KULeuven/Common/EndHeader}} | ||
{{Team:KULeuven/Notebook/DayNavigator}} | {{Team:KULeuven/Notebook/DayNavigator}} | ||
Revision as of 09:05, 4 September 2009
Project progress
Progress of parts
[edit] Blue Light Receptor
PCR reaction with the new primers 2260 and 2261 to get the exact blue light promotor sequence.
The reaction was succesful and with no byproducts, so a simple PCR clean up was executed.
- concentration of clean pcr product.:
- 102,6 ng/µl
- 1,88
[edit] Vanillin Production
- Ligated genes are electrophoresed.
- Reorderd part (COMT + RBS) from the registry.
- Also we realized the proteins need to be degraded fast for the control mechanism to work, so a ssRA degradation tag like ANDENYALVA or ANDENYALAA is needed.
- However since some genes have just been ligated we will only tag sam5, COMT and the ech gene.
[edit] Vanillin Receptor
- There was no result from our setup on Friday. We think there was an error with the protocol because we didn't shake before incubation. So we did a new transformation of every gene.
