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Team:KULeuven/Modelling/Vanillin Production
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== overview == | == overview == | ||
[[image:Vanillin_Biosynthesis_Pathway.jpg|400px|right|Vanillin production overview starting from Tyrosine.]] | [[image:Vanillin_Biosynthesis_Pathway.jpg|400px|right|Vanillin production overview starting from Tyrosine.]] | ||
| - | The vanillin synthesis consists of a five step process starting from tyrosine. | + | |
| + | The vanillin synthesis consists of a five step process starting from tyrosine. By locking both the transcription of the Sam 8 and COMT enzyme we prevent vanillin synthesis without the presence of RIBOKEY. E. coli controls its own tyrosine production which is an non essential amino acid. However,should this be insufficient, we can always add extra tyrosine. The production process will be tested in two steps. First, from tyrosine to ferulic acid; then from ferrulic acid to vanillin. | ||
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[[Image:Biologie_vanillin_synthesis.png|center]] | [[Image:Biologie_vanillin_synthesis.png|center]] | ||
[[Image:Biologie_vanillin_synthesis_short.png|center]] | [[Image:Biologie_vanillin_synthesis_short.png|center]] | ||
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==Models== | ==Models== | ||
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[[image:Vanillin_production.png|750px|center]] | [[image:Vanillin_production.png|750px|center]] | ||
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| + | ==Simulation== | ||
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| + | Because COMT and SAM8 are regulated by the amount of mRNA key its worthy to investigate the change of COMT and SAM8 production in function of the input amount of mRNA key. | ||
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| + | [[image:COMT.png|750px|center|thumb|Concentration of COMT in function of time and input key]] | ||
Revision as of 14:35, 4 September 2009
Contents |
Vanillin Production
overview
The vanillin synthesis consists of a five step process starting from tyrosine. By locking both the transcription of the Sam 8 and COMT enzyme we prevent vanillin synthesis without the presence of RIBOKEY. E. coli controls its own tyrosine production which is an non essential amino acid. However,should this be insufficient, we can always add extra tyrosine. The production process will be tested in two steps. First, from tyrosine to ferulic acid; then from ferrulic acid to vanillin.
Models
Simulation
Because COMT and SAM8 are regulated by the amount of mRNA key its worthy to investigate the change of COMT and SAM8 production in function of the input amount of mRNA key.
