Uppsala-Sweden/6 September 2009

From 2009.igem.org

(Difference between revisions)
(Colony PCR)
(Picking Colonies)
Line 13: Line 13:
--[[User:Karl.brune|Karl.brune]] 23:08, 5 September 2009 (UTC)
--[[User:Karl.brune|Karl.brune]] 23:08, 5 September 2009 (UTC)
5 Colonies of pirA (AC3) -> pirA-X
5 Colonies of pirA (AC3) -> pirA-X
 +
5 Colonies of b0034-pirA (AC3) -> RA-X
5 Colonies of b0034-pirA (AC3) -> RA-X
 +
5 Colonies of B0034-Yadh2-B0015 (AC3) -> RYB-X
5 Colonies of B0034-Yadh2-B0015 (AC3) -> RYB-X
 +
5 Colonies of B0015 (AC3) - T-X
5 Colonies of B0015 (AC3) - T-X
 +
5 Colonies of R0011-B0034 (AK3) -> RR-X
5 Colonies of R0011-B0034 (AK3) -> RR-X
 +
3 Colonies of E1A1-B0015 (AK3) -> E1A1X
3 Colonies of E1A1-B0015 (AK3) -> E1A1X
 +
3 Colonies of E1A2-B0015 (AK3) -> E1A2X
3 Colonies of E1A2-B0015 (AK3) -> E1A2X
 +
3 Colonies of E1A3-B0015 (AK3) -> E1A3X
3 Colonies of E1A3-B0015 (AK3) -> E1A3X
 +
3 Colonies of E1A4-B0015 (AK3) -> E1A4X
3 Colonies of E1A4-B0015 (AK3) -> E1A4X
 +
3 Colonies of E1B1-B0015 (AK3) -> E1B1X
3 Colonies of E1B1-B0015 (AK3) -> E1B1X
 +
3 Colonies of E1B2-B0015 (AK3) -> E1B2X
3 Colonies of E1B2-B0015 (AK3) -> E1B2X
 +
3 Colonies of E1B3-B0015 (AK3) -> E1B3X
3 Colonies of E1B3-B0015 (AK3) -> E1B3X
 +
3 Colonies of E1B4-B0015 (AK3) -> E1B4X
3 Colonies of E1B4-B0015 (AK3) -> E1B4X
 +
3 Colonies of E21-B0015 (AK3) -> E21X
3 Colonies of E21-B0015 (AK3) -> E21X
 +
3 Colonies of E22-B0015 (AK3) -> E22X
3 Colonies of E22-B0015 (AK3) -> E22X
 +
3 Colonies of E23-B0015 (AK3) -> E23X
3 Colonies of E23-B0015 (AK3) -> E23X
 +
3 Colonies of E24-B0015 (AK3) -> E24X
3 Colonies of E24-B0015 (AK3) -> E24X
 +
X= # of colony
X= # of colony

Revision as of 23:09, 5 September 2009

Contents

Moving ON to fride

ON cultures for the lux system were moved to the iGEM fridge for MiniPrep tomorrow

Checking Plates

All plates show colonoies. After picking colonies -> fridge. --Karl.brune 23:08, 5 September 2009 (UTC)

Picking Colonies

I picked colonies from plates into 10µl selective media. --Karl.brune 23:08, 5 September 2009 (UTC) 5 Colonies of pirA (AC3) -> pirA-X

5 Colonies of b0034-pirA (AC3) -> RA-X

5 Colonies of B0034-Yadh2-B0015 (AC3) -> RYB-X

5 Colonies of B0015 (AC3) - T-X

5 Colonies of R0011-B0034 (AK3) -> RR-X


3 Colonies of E1A1-B0015 (AK3) -> E1A1X

3 Colonies of E1A2-B0015 (AK3) -> E1A2X

3 Colonies of E1A3-B0015 (AK3) -> E1A3X

3 Colonies of E1A4-B0015 (AK3) -> E1A4X


3 Colonies of E1B1-B0015 (AK3) -> E1B1X

3 Colonies of E1B2-B0015 (AK3) -> E1B2X

3 Colonies of E1B3-B0015 (AK3) -> E1B3X

3 Colonies of E1B4-B0015 (AK3) -> E1B4X


3 Colonies of E21-B0015 (AK3) -> E21X

3 Colonies of E22-B0015 (AK3) -> E22X

3 Colonies of E23-B0015 (AK3) -> E23X

3 Colonies of E24-B0015 (AK3) -> E24X


X= # of colony

--Karl.brune 23:08, 5 September 2009 (UTC)

Colony PCR

TAQ colony PCR was performed.

10µl reaction, standart mix with verfication primer VF2 and VR.

Initial Denat: 1min 30sec @94°C

40Cylces

Denaturation: 45 sec @94°C

Annealing: 45 sec @50°C

Elongation: 1min 45sec @ 72°C

Return to Denat.

Final Elongation: 10min @72°C

Infinite storage at 4°C

Colony tubes were moved to fridge after taking template for the PCR

--Karl.brune 23:08, 5 September 2009 (UTC)