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Team:Stanford/Project Homeostasis/sequences
From 2009.igem.org
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Revision as of 09:08, 6 September 2009
| Project |
| Research Proposal |
| Systems Overview |
| Cloning Plan |
| Sequences & Primers |
| Anti-Inflammation |
| Device Overview |
| Parts Design |
| Challenges |
| Results |
| Anti-Immunosuppression |
| Device Overview |
| Parts Design |
| Challenges |
| Results |
| Protocols |
| Modeling |
| Overview |
| Notebook |
| Results |
| Future Work |
| Archived Work |
Contents |
Device 1
RBS – SoxR gene – Terminator – Spacer – SoxS Promoter click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/SoxRS here]
SoxR gene click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/SoxR here]
SoxS Promoter click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/SoxSPromoter here]
RBS - Blh gene click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/Blh here]
RBS - RALDH II gene - Terminator click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/RaldhII here]
hIL-6 hlyAs click [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/sequences/Il6HlyAs here]
Primer Design Guidelines
- Primer Design
- [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/Helpful_Websites Guidelines to Create Primers]
- Designing Mutagenic Primers (see page 10)
Resources
- National Center for Biotechnology Information (NCBI)- Get Your Sequences
[http://www.ncbi.nlm.nih.gov/ Home Page]
- Parts Registry of BioBricks- Help with Prefix and Suffix
[http://partsregistry.org/Help:BioBrick_Prefix_and_Suffix Prefix and Suffix Help]
- IDT Integrated DNA Technologies- Check for %GC (40-60) and Melting Temp. (52-68C)
[http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ Analyze Your Sequence]
- NUPACK Nucleic Acid Package- check both primers for hairpins and trouble spots (No Red!!)
[http://nupack.org/partition/new Analzye both Primers]
- ApE- A Plasmid Editor- Useful tool for checking sequences and finding reverse complements
[http://www.biology.utah.edu/jorgensen/wayned/ape/ Download here]
- Ginkgo BioWorks- creates primers w/ BioBrick additions
[http://ginkgobioworks.com/cgi/primer.cgi Design Primers Here]
Archived Documents and Work
- Past Primers and Sequences
- [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/Primer/Primersdoc Finalized Original Primers]
- [http://openwetware.org/wiki/IGEM:Stanford/2009/Project_Homeostasis/Primer Original Primers]
