Team:UNIPV-Pavia/Freezer

From 2009.igem.org

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Revision as of 07:02, 8 September 2009

EthanolPVanimation.gif

Biobrick/
Ligation Name		 Description Creation Date N° usage Strain Vector Quality Control
J23100(J100)
replaced by "J23100 Jun4"		 constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23101(J101)
replaced by "J23101 Jun4"		 constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23102(J102) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23103(J103) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23104(J104) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23105(J105) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23106(J106) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23110(J110) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23112(J112) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23113(J113) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23114(J114) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23116(J116) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23117(J117) constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
J23118(J118)
replaced by "J23118 Jun4"		 constitutive promoter May 25 0 TOP10 J61002(Amp) sequence ok (MIT)
E0240(GFP3) GFP protein generator
(RBS-GFP-TT)		 May 25 5 TOP10 pSB1A2 length ok (PCR) sequence ok (MIT)
K131009(E2) colE2 operon:ceaB-ceiB-Tweak-celB-T (with RBS) May 28 1 TOP10 pSB1AK3 sequence BAD in prefix,suffix,celB (MIT,BMR)
K117000(celB) colE7 lysis gene May 28 1 TOP10 pSB1A2 sequence ok (MIT)
K124017(lysis21) Phage Lysis Cassette May 28 0 TOP10 pSB1AK3 sequence BAD (MIT)
B0015(TT) Double terminator May 28 3 TOP10 pSB1AK3 sequence ok (MIT)
I732017 (RBS-lacZ) RBS-lacZ May 28 1 TOP10 pSB1A2 long part (MIT)
B0030 RBS 0.6 Jun 4 1 TOP10 pSB1A2 sequence ok (MIT,BMR)
B0031 RBS 0.07 Jun 4 0 TOP10 pSB1A2 sequence ok (MIT)
B0032 RBS 0.3 Jun 4 0 TOP10 pSB1A2 sequence ok (MIT)
B0033 RBS 0.01 Jun 4 0 TOP10 pSB1A2 sequence ok (MIT)
B0034 RBS 1 Jun 4 0 TOP10 pSB1A2 sequence ok (MIT)
P0440(P04) RBS-tetR-TT Jun 4 1 TOP10 pSB1A2 length ok (PCR) sequence ok (MIT)
R0040(Ptet) Ptet Jun 4 1 TOP10 pSB1A2 sequence ok (MIT)
T9002 Ptet-RBS-luxR-TT-Plux-RBS-GFP-TT Jun 4 2 TOP10 pSB1A3 sequence BAD (MIT) - sequence ok (BMR)
J23100(J100 Jun4) constitutive promoter Jun 4 3 TOP10 J61002(Amp) sequence ok (MIT,BMR)
J23101(J101 Jun4) constitutive promoter Jun 4 2 TOP10 J61002(Amp) sequence ok (MIT)
J23118(J118 Jun4) constitutive promoter Jun 4 3 TOP10 J61002(Amp) sequence ok (MIT)
A1 J23100(Jun 4)-E0240
(Pcon-RBS-GFP-TT)		 Jun 11 3 TOP10 pSB1A2 length ok (PCR)
A2 J23101(Jun 4)-E0240
(Pcon-RBS-GFP-TT)		 Jun 11 1 TOP10 pSB1A2 length ok (PCR)
A3-1 I732017-B0015
(RBS-lacZ-TT)		 Jun 12 2 TOP10 pSB1AK3 length ok (digestion PstI) sequence ok -long part- (BMR)
A4 P0440-R0040
(RBS-tetR-TT-Ptet)		 Jun 11 2 TOP10 pSB1A2 length ok (PCR) sequence ok (BMR)
A5 A4-A3
(RBS-tetR-TT-Ptet-RBS-lacZ-TT)		 Jun 19 0 TOP10 pSB1AK3 sequence ok -long part- (BMR)
A6 J23100(Jun 4)-A4
(Pcon-RBS-tetR-TT-Ptet)		 Jun 19 1 TOP10 pSB1A2 length ok (PCR) sequence ok (BMR)
BOL1 J23118-B0034-C0012-B0015
(Pcon-RBS-lacI-TT)		 Jun 23 3 TOP10 pSB1A2 sequence ok (BMR)
A7 J23118(Jun 4)-E0240
(Pcon-RBS-GFP-TT)		 Jun 25 1 TOP10 pSB1A2 length ok (PCR)
R0011(Plac) Plac Jun 25 1 TOP10 pSB1A2 sequence ok (MIT)
K112808(ENTERO) holin-lysozyme-T-Pconweak-antiholin-T (with RBSs) Jun 25 1 TOP10 pSB1A2 sequence almost ok -long part- (MIT): it has several gaps in the scars, a silent point mutation in holin gene and a wrong annotated terminator at the end of the brick (that is actually K112710, not B0010).
A8 A4-E0240
(RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jun 29 1 TOP10 pSB1A2 NON PURE PLASMID! (digestion E-P) but works
A9 A6-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jun 29 1 TOP10 pSB1A2 NON PURE PLASMID! (digestion E-P) but works
A10
thrown away		 K117000-B0015
(celB-TT)		 Jun 30 2 TOP10 pSB1AK3 sequence BAD (BMR)
A8pg A4-E0240
(RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jul 3 1 TOP10 pSB1A2 length ok (digestion E-P)
A9pg A6-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Jul 3 1 TOP10 pSB1A2 length ok (digestion E-P)
A11-1
to throw away!		 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Jul 10 1 TOP10 pSB1A2 length ok (PCR)
sequence MUTATED
A11-3
to throw away!		 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Jul 10 0 TOP10 pSB1A2 length ok (PCR)
A11-6
to throw away!		 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Jul 10 0 TOP10 pSB1A2 length ok (PCR)
F2620
to throw away!		 Ptet-RBS-luxR-TT-Plux
 Jul 10 1 TOP10 pSB1A2 sequence BAD (MIT, BMR)
A12-2 J23118(Jun 4)-A4
 Jul 16 1 TOP10 pSB1A2 length ok (PCR)
sequence ok (BMR)
A12-3 J23118(Jun 4)-A4
 Jul 16 1 TOP10 pSB1A2 length ok (PCR)
sequence ok (BMR)
A14-4
to throw away!		 A11-E0240
 Jul 16 0 TOP10 pSB1A2 NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR)
A14pg
to throw away!		 A11-E0240
 Jul 16 1 TOP10 pSB1A2 NON PURE PLASMID! (digestion) and the A11 sample used for ligation was MUTATED (BMR)
K116002 (PnhaA+GFP) PnhaA-RBS-GFP-TT
 Jul 29 0 ? pSB1A2 sequence almost ok (BMR): inconsistent prefix (but restriction sites are good) and additional "c" at the end of promoter
K112405 (Pcad) cadAB promoter
 Jul 29 0 ? K112951(Amp+Cm) sequence ok (BMR)
P0412 RBS-lacI-TT
 Jul 29 0 ? pSB1A2 sequence ok (BMR)
I746902 GFPmut3+his tag generator under Pbad
 Jul 29 0 ? pSB1AK3 sequence ok -long part- (BMR)
I746903 GFPmut3+his tag generator under PT7
 Jul 29 0 ? pSB1AK3 sequence almost ok (BMR): the RBS is B0030 and not B0034 as documented, but the function is the same
K101017
to throw away!		 MioC promoter
 Jul 29 0 ? pSB1A2 sequence BAD (BMR)
F2620MIT1 Ptet-RBS-luxR-TT-Plux
 Jul 29 1 ? pSB1A2 sequence ok (BMR)
F2620MIT2 Ptet-RBS-luxR-TT-Plux
 Jul 29 0 ? pSB1A2 sequence ok (BMR)
MRGENE1 pdc Jul 17 0 TOP10 pMK-RQ
(Kan)		 antibiotic ok
length ok (digestion X-P)
sequence ok (Mr Gene)
MRGENE1-2 pdc Jul 17 0 TOP10 pMK-RQ
(Kan)		 antibiotic ok
sequence ok (Mr Gene)
MRGENE1-3 pdc Jul 17 0 TOP10 pMK-RQ
(Kan)		 antibiotic ok
sequence ok (Mr Gene)
MRGENE2 adhB Jul 17 0 TOP10 pMA
(Amp)		 antibiotic ok
length ok (digestion X-P)
sequence ok (Mr Gene)
MRGENE2-2 adhB Jul 17 0 TOP10 pMA
(Amp)		 antibiotic ok
sequence ok (Mr Gene)
MRGENE2-3 adhB Jul 17 0 TOP10 pMA
(Amp)		 antibiotic ok
sequence ok (Mr Gene)
B1-13 B0030-MRGENE1
(RBS-pdc)		 Jul 28 1 TOP10 pSB1A2 length ok (digestion E-P
, digestion S-P and digestion E-S)
sequence ok (BMR)
B2-5 B0030-MRGENE2
(RBS-adhB)		 Jul 23 2 TOP10 pSB1A2 length ok (PCR
and digestion S-P)
sequence ok (BMR)
B3-5 B1-B0015
(RBS-pdc-TT)		 Jul 31 1 TOP10 pSB1AK3 length ok+extra band (digestion XhoI-P
and digestion X-P)
sequence ok (BMR)
B4-2 B2-B0015
(RBS-adhB-TT)		 Jul 31 1 TOP10 pSB1AK3 length ok (digestion XhoI-P)
sequence ok (BMR)
B5-3 B1-B4
(RBS-pdc-RBS-adhB-TT)		 Aug 12 1 TOP10 pSB1AK3 length ok+2 extra bands (digestion E-P)
sequence ok (BMR)
B6-3 B2-B3
(RBS-adhB-RBS-pdc-TT)		 Aug 12 1 TOP10 pSB1AK3 length ok+2 extra bands (digestion E-P)
sequence ok (BMR)
A11-2
to throw away!		 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Aug 13 1 TOP10 pSB1A2 length ok (digestion S-P) sequence MUTATED (BMR)
A11-3 BOL1-R0011
(Pcon-RBS-lacI-TT-Plac)		 Aug 13 0 TOP10 pSB1A2 length ok (digestion S-P) sequence ok (BMR)
A15-1
thrown away by mistake...		 F2620MIT1-K112808
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)		 Aug 14 0 TOP10 pSB1A2 length ok (digestion) sequence ok (BMR)
A15-2 F2620MIT1-K112808
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)		 Aug 14 1 TOP10 pSB1A2 length ok (digestion) sequence WRONG (BMR)
A15-3 F2620MIT1-K112808
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)		 Aug 14 0 TOP10 pSB1A2 length ok (digestion) sequence ok (BMR)
B8-5
thrown away!		 A4-B6
(RBS-tetR-TT-Ptet-RBS-adhB-RBS-pdc-TT)		 Aug 21 1 TOP10 pSB1AK3 length maybe ok () sequence WRONG (BMR)
A14-3
to throw away!		 A11(-2 Aug13)-E0240
(Pcon-RBS-lacI-TT-Plac-RBS-GFP-TT)		 Aug 21 0 TOP10 pSB1A2 length ok (PCR) sequence of A11-2(Aug13) was MUTATED (BMR)
A16-4 A12-E0240
(Pcon-RBS-tetR-TT-Ptet-RBS-GFP-TT)		 Aug 21 0 TOP10 pSB1A2 length ok (PCR) sequence ok (BMR)
A17-4
to throw away! (Wait, are we sure?)		 R0011-E0240
(Plac-RBS-GFP-TT)		 Aug 28 0 TOP10 pSB1A2 length ok (PCR) sequence WRONG! ligation failed.
A18-? A15(-2)-pSB3K3
(Ptet-RBS-luxR-TT-Plux-RBS-holin-RBS-lysozyme-T-Pconweak-RBS-antiholin-T)		 Aug 28 0 TOP10 pSB3K3 still to screen

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