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Template:Team:KULeuven/10 August 2009/VanillinProduction
From 2009.igem.org
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* Reorderd part {{kulpart|BBa_I742109}} (COMT + RBS) from the registry. | * Reorderd part {{kulpart|BBa_I742109}} (COMT + RBS) from the registry. | ||
* Also we realized the proteins need to be degraded fast for the control mechanism to work, so a ssRA degradation tag like ANDENYALVA or ANDENYALAA is needed. | * Also we realized the proteins need to be degraded fast for the control mechanism to work, so a ssRA degradation tag like ANDENYALVA or ANDENYALAA is needed. | ||
| - | ** However since some genes have just been ligated we will only tag Sam5, | + | ** However since some genes have just been ligated we will only tag Sam5, COMT and the Ech gene. |
Revision as of 08:38, 8 September 2009
- Ligated genes are electrophoresed.
- Reorderd part (COMT + RBS) from the registry.
- Also we realized the proteins need to be degraded fast for the control mechanism to work, so a ssRA degradation tag like ANDENYALVA or ANDENYALAA is needed.
- However since some genes have just been ligated we will only tag Sam5, COMT and the Ech gene.
