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Team:Warsaw/Calendar-Main/1 September 2009
From 2009.igem.org
(Difference between revisions)
(New page: {{WarNotebook}} <!-- do not edit above me! --> <html><h3><div style="text-align: center;">Making of the plac-RBS-llo-intA part</div></h3> <h4>Jarek</h4> <br /> <p>Tasks:</p> <ul> <li>Isola...) |
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<li>Isolation of plasmid DNA from liquid cultures.</li> | <li>Isolation of plasmid DNA from liquid cultures.</li> | ||
<li>Digestion of DNA samples with EcoRI and PstI endonucleases.</li> | <li>Digestion of DNA samples with EcoRI and PstI endonucleases.</li> | ||
| + | </ul> | ||
| + | <br/> | ||
| + | |||
| + | <h3><div style="text-align: center;">Cloning of the mgtc promoter into the pSB1A3 plasmid</div></h3> | ||
| + | <h4>Kamil</h4> | ||
| + | <br /> | ||
| + | <p>Tasks:</p> | ||
| + | <ul> | ||
| + | <li>Bacteria transformation</li> | ||
| + | </ul> | ||
| + | <br /> | ||
| + | <p>Methods:</p> | ||
| + | <ul> | ||
| + | <li>A fresh batch of chemocompetent bacteria was transformed with the ligation mix and incubated on agarose plates containing double dose of ampicilin</li> | ||
| + | </ul> | ||
| + | <br /> | ||
| + | |||
| + | <h3><div style="text-align: center;">Cloning of the cro-box into the pSB1A3 plasmid</div></h3> | ||
| + | <h4>Kamil</h4> | ||
| + | <br /> | ||
| + | <p>Tasks:</p> | ||
| + | <ul> | ||
| + | <li>Bacteria transformation</li> | ||
| + | </ul> | ||
| + | <br /> | ||
| + | <p>Methods:</p> | ||
| + | <ul> | ||
| + | <li>A fresh batch of chemocompetent bacteria was transformed with the ligation mix and incubated on agarose plates containing double dose of ampicilin</li> | ||
| + | </ul> | ||
| + | <br /> | ||
| + | |||
</html> | </html> | ||
<!-- do not remove this! --> | <!-- do not remove this! --> | ||
{{WarNotebookEnd}} | {{WarNotebookEnd}} | ||
Revision as of 21:08, 8 September 2009
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Making of the plac-RBS-llo-intA part
Jarek
Tasks:
- Isolation of plasmid DNA from liquid cultures.
- Digestion of DNA samples with EcoRI and PstI endonucleases.
Cloning of the mgtc promoter into the pSB1A3 plasmid
Kamil
Tasks:
- Bacteria transformation
Methods:
- A fresh batch of chemocompetent bacteria was transformed with the ligation mix and incubated on agarose plates containing double dose of ampicilin
Cloning of the cro-box into the pSB1A3 plasmid
Kamil
Tasks:
- Bacteria transformation
Methods:
- A fresh batch of chemocompetent bacteria was transformed with the ligation mix and incubated on agarose plates containing double dose of ampicilin
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